Detailed information of TA system TA188218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3678297..3679117
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: JNN61_RS17510
• Protein ID: WP_001550554.1
• Coordinates: 3678860..3679117 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: JNN61_RS17505
• Protein ID: WP_001519303.1
• Coordinates: 3678297..3678848 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS17470	3673992..3675305	-	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-
JNN61_RS17475	3675317..3675595	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JNN61_RS17480	3675592..3676713	-	1122	WP_000010833.1	M42 family metallopeptidase	-
JNN61_RS17485	3676958..3677074	-	117	Protein_3411	VOC family protein	-
JNN61_RS17490	3677112..3677372	-	261	WP_000084876.1	hypothetical protein	-
JNN61_RS17495	3677499..3678047	-	549	WP_001519301.1	hypothetical protein	-
JNN61_RS17500	3678084..3678245	-	162	WP_001519302.1	hypothetical protein	-
JNN61_RS17505	3678297..3678848	-	552	WP_001519303.1	N-acetyltransferase	Antitoxin
JNN61_RS17510	3678860..3679117	-	258	WP_001550554.1	hypothetical protein	Toxin
JNN61_RS17515	3679494..3679793	-	300	WP_001519304.1	GNAT family N-acetyltransferase	-
JNN61_RS17520	3679856..3681097	+	1242	Protein_3418	helicase YjhR	-
JNN61_RS17525	3681680..3682660	-	981	WP_001519307.1	transposase	-
JNN61_RS17530	3682725..3683831	-	1107	WP_001519308.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	3678297..3690102	11805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T188218 WP_001550554.1 NZ_CP068812:c3679117-3678860 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T188218 NZ_CP090366:1548178-1548492 [Stutzerimonas kunmingensis]
ATGATCTTTATCGAGACACCGATCTTCTCCAAGCGTCTGCGGGAGCTGCTTAGCGATGACAGCTATGCGGAGTTTCAGCG
GCAACTGGCCGACCGGCCGGACATGGGCGATGTGATTGAAGGTACTGGCGGCATTCGCAAGGTTCGCGTTGCGTCTGGTG
GTCACGGCAAGCGAGGCGGGTCCAGGGTCATTTACTACCACTTCACGGCGGCTTCGCAGATCGCATTACTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGACGAGCGCAAGGTGCTCAAGCAAATCATCGAACGGTGGAGGTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT188218 WP_001519303.1 NZ_CP068812:c3678848-3678297 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT188218 NZ_CP090366:1548498-1548788 [Stutzerimonas kunmingensis]
ATGAGCAAATTCTTCGAAGACCTTCTGGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAACGTCAGCCCTCGCG
GGAATTCGTCGTTGACTCGCTGCAGGTGAAAGAGATCCGCAAGGCGACCGGGCTGACCCAGGCCAAATTCGCAGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGCCGACGCGAACCGACCGGACCAGCAAAGGCCCTGCTGCGC
GCCATCCATAACGATCCGAAGCACGTCATCCAGGCGCTATCGTCCGTCTGA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8