Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3395241..3395738 Replicon chromosome
Accession NZ_CP068812
Organism Escherichia coli strain RIVM_C029020

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN61_RS16055 Protein ID WP_001173343.1
Coordinates 3395565..3395738 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN61_RS16050 Protein ID WP_000398619.1
Coordinates 3395241..3395537 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN61_RS16030 3391971..3392690 - 720 WP_000611288.1 two-component system response regulator DcuR -
JNN61_RS16035 3392687..3394318 - 1632 WP_001216477.1 sensor histidine kinase -
JNN61_RS16040 3394498..3394728 + 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN61_RS16045 3394740..3395012 + 273 WP_000405647.1 N-acetyltransferase -
JNN61_RS16050 3395241..3395537 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN61_RS16055 3395565..3395738 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN61_RS16060 3395857..3397374 - 1518 WP_001550472.1 lysine--tRNA ligase -
JNN61_RS16065 3397611..3399068 - 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T188214 WP_001173343.1 NZ_CP068812:3395565-3395738 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T188214 NZ_CP090357:c266554-266087 [Pantoea ananatis]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCGATGGTCTACGCTTCTGAAACCGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTGCTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTTCCTGGCGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTTCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCCGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAACATCCCCTTCCTTTTTGACGCACGTTTTTCCCGACAGGCATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT188214 WP_000398619.1 NZ_CP068812:3395241-3395537 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT188214 NZ_CP090357:c266994-266551 [Pantoea ananatis]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTATGGCAATTTGCTGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGACGGCACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAAAGCCGCTTTACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAGGGCGATAAAGAAAAGGCACGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTAGGCAATAAAAAACCGGCTGATCTGGTCGCGTCAGAAACCGGGGCTTACGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGTGTGTATTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References