Detailed information of TA system TA188211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2639400..2640012
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JNN61_RS12575
• Protein ID: WP_000833473.1
• Coordinates: 2639827..2640012 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JNN61_RS12570
• Protein ID: WP_000499744.1
• Coordinates: 2639400..2639810 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS12555	2634912..2635727	-	816	WP_000891823.1	AraC family transcriptional regulator	-
JNN61_RS12560	2635953..2637353	+	1401	WP_000204811.1	MFS transporter	-
JNN61_RS12565	2637364..2639328	+	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
JNN61_RS12570	2639400..2639810	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JNN61_RS12575	2639827..2640012	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JNN61_RS12580	2640281..2641819	-	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
JNN61_RS12585	2642020..2643171	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T188211 WP_000833473.1 NZ_CP068812:c2640012-2639827 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T188211 NZ_CP090356:c3896154-3895762 [Pantoea ananatis]
ATGGGTTTTACGCTTTATGTTCATAAACGTTTTGACAGAGTGTTTAAAAAAACGCGTCTGACAGACAGCGTCCTGTGTTA
CGCAGCAGGTGACGTTTTTACTGGCGTGTTTGAGGCTGACTTAGGCGGTGGCGTTATTAAAAAGCGTCTTGCTCTTCACC
AAGGCAAGCGTGGCGGCGCGCGAACGGTCATCTTCTTCAGACAAGGCACCAACCTGGTTTATTACGATGGATGGGTCAAA
AACTTGTCGAGGAAATACGCCAGGGAAATCGATGAAGATCAGCTGGATACCTACAGGGATGTGGCAAGAATGTTTTTCCG
TGCAAATAGTGAAGTGATGAGCCTGCTCGTCAGAAGCGGAATGGTCAGAGAGGTTAAATGTAATGAAAACTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT188211 WP_000499744.1 NZ_CP068812:c2639810-2639400 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT188211 NZ_CP090356:c3895772-3895419 [Pantoea ananatis]
ATGAAAACTGAACCCGCCGAATTGAGTGAACTACTTGAAGATGCGAAAGCGTTTCACGCGTTAGGACTGGCAACAGATGA
AGAAGTGGAGTCCATAGCAAAGCGCGCAAAAAATCGTGAACTCCGTGCCCGTATTGCATCAGTAAAGCCCATGACAGCAA
CTGAAATCAAAGACGTGCGCATGCGTTGGGGAATGTCGCAATCGCTTGTGGCGCTCTCATTTGGCATGACGGTCGATAGC
GTATCGAAATGGGAGCGAGGTGAAATCACTCCGAGCCCACCGGTGATGCGGATGCTGCAACTCCTTCGCGATAACGGCCC
TGAACTCTTTACCTCTAAGCATGAAGGTATCTAG

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9