Detailed information of TA system TA188209

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1961137..1961971
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: JNN61_RS09295
• Protein ID: WP_000854690.1
• Coordinates: 1961594..1961971 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: JNN61_RS09290
• Protein ID: WP_001305076.1
• Coordinates: 1961137..1961505 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS09250	1956220..1957347	+	1128	Protein_1811	hypothetical protein	-
JNN61_RS09255	1957423..1957878	+	456	WP_000581502.1	hypothetical protein	-
JNN61_RS09260	1957957..1958190	+	234	WP_001119729.1	DUF905 family protein	-
JNN61_RS09265	1958290..1959108	+	819	WP_001234628.1	DUF945 domain-containing protein	-
JNN61_RS09270	1959163..1959648	+	486	WP_000849565.1	antirestriction protein	-
JNN61_RS09275	1959664..1960140	+	477	WP_001186726.1	RadC family protein	-
JNN61_RS09280	1960203..1960424	+	222	WP_000692329.1	DUF987 domain-containing protein	-
JNN61_RS09285	1960443..1961087	+	645	WP_000094916.1	hypothetical protein	-
JNN61_RS09290	1961137..1961505	+	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN61_RS09295	1961594..1961971	+	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
JNN61_RS09300	1961968..1962456	+	489	WP_000761699.1	hypothetical protein	-
JNN61_RS09305	1962473..1962670	+	198	WP_000839293.1	DUF957 domain-containing protein	-
JNN61_RS09310	1962755..1963600	+	846	WP_001529401.1	DUF4942 domain-containing protein	-
JNN61_RS09315	1963669..1964064	+	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
JNN61_RS09320	1964057..1964990	+	934	Protein_1825	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
JNN61_RS09325	1965422..1965577	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1965422..1965577	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T188209 WP_000854690.1 NZ_CP068812:1961594-1961971 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T188209 NZ_CP090356:c3559256-3558147 [Pantoea ananatis]
ATGGCAAAGCTCATTGCCTGGATAAACGGCGAGCGTACGGGAACGCTGACGCTGCATCACAACGGCGCGCTGCATTTCCA
GTATGACGCCAACTGGCTTCAGCATCCGCTTGCCAGACCTCTCTCGCTTTCGTTGCCCTTACAGCGCGCCGCGATGCATT
CTGAGAAAGTCGTGCACTTTTTCGAAAACCTTCTCTATCCCAGTACGTTGATCGAAAAGGATTTTTACCCGACTCGCCCA
ATAAAATCCTGTGAGGTGTTTGCTGCATTGCAGAAAGTGGGACACACATTACCCGGGGCGCTCAGTCTGCTGCCGGCAAA
GCGGCAGCCCGTCGCGCCGGACCAGGCAACGCACAGCGTCTTCAGCCCCGCCGATGAGGCACAGCAGTTCGATATCGATA
ATGCGTATCTGTGTCTGTCCCTGGCCCGGGAGCTGACTATCCCGGTGCCGGATGCCGTGTTAGTTCATGAGAACGGCATC
CGTAAATTGAGAACAGAACGATACGATCGCCGTTGGCGTCACGACGGACAACACCTTTATCATCTTCCTGAAGAAGACGT
GTGTCAGGCGCTCGGCGTGCCGCTCTCTGGCCGCGATGAGCAGCAGGGCGGTCCGGGAATTGATGACATCATGGCCTTGC
TGATGGGCTCAAGCTCCGCCATGAAAGACCGTGAGGATATGATGCGCTGTCTGGTATTTCAGTGGCTGACGGGCATCACG
CGCGTCAACGCTAAACATCTCTCGCTGCTGATTGCTCGCGGTGGCAGCTTCCGGCTCGCGCCTTTTAAGCATCTCCTTTC
CGCCTGCCCGGGCCTTTCTCTACGCACAACAGACAGACGGGATTTCCAGCTGGGGATGGGCCTGACAGGCCCTGAGGGCA
AACAGCATCACATTGACGCGATTGGGCCCGCGCATTTTCTTGCCCTTGCGGGGAGCGTGGGGTTCAGTCAGGGATCGATG
CGGCAGATACTGGCGGCCTTTATTCCGGGCTTGCCTGCCGCGATTGTTAAGGTGCGGGACAGCCTGCCCGATGATTATCC
CCTGACGCTCGCCGATGCGGTTTTCAGCCACGCAATGATGATGCTGGAAAGGGTGCGCCAGGCGATCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT188209 WP_001305076.1 NZ_CP068812:1961137-1961505 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188209 NZ_CP090356:c3559495-3559256 [Pantoea ananatis]
ATGATTTACAGTCCCCGCCAGTTGGCGAATCAGGTCAGGCTATTGCGGCAGCAAAACGGCTGGACCCAGGTCGAACTGGC
GAAAAAAGTGGGCCTGAAACAGGCAACCCTGTCTCACTTCGAAAATGCCCCGGATACGACCTCACTGGCAACGTTGTTCA
AAATCTTACAGGCGCTCGATCTTGCTCTGTCGCTTCATGAAAACAGCGCATCCTCAGCCCCGGCTGAGGATGCCTGGTAA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8