Detailed information of TA system TA188204

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 895656..896487
• Replicon: chromosome
• Accession: NZ_CP068812
• Organism: Escherichia coli strain RIVM_C029020

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6C9I6Z5
• Locus tag: JNN61_RS04370
• Protein ID: WP_001563930.1
• Coordinates: 896113..896487 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN61_RS04365
• Protein ID: WP_001285585.1
• Coordinates: 895656..896024 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN61_RS04335	892545..893000	+	456	WP_000581502.1	hypothetical protein	-
JNN61_RS04340	893079..893312	+	234	WP_001119729.1	DUF905 family protein	-
JNN61_RS04345	893412..894230	+	819	WP_023910202.1	DUF945 domain-containing protein	-
JNN61_RS04350	894321..894806	+	486	WP_000214393.1	antirestriction protein	-
JNN61_RS04355	894822..895298	+	477	WP_023910200.1	RadC family protein	-
JNN61_RS04360	895361..895582	+	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN61_RS04365	895656..896024	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN61_RS04370	896113..896487	+	375	WP_001563930.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN61_RS04375	896484..896678	+	195	WP_000988600.1	hypothetical protein	-
JNN61_RS04380	896691..896804	+	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN61_RS04385	897093..897221	-	129	Protein_863	transposase domain-containing protein	-
JNN61_RS04390	897293..897475	+	183	WP_001016348.1	hypothetical protein	-
JNN61_RS04395	897576..897905	-	330	WP_000450409.1	DUF496 family protein	-
JNN61_RS04400	898077..899135	-	1059	WP_001200891.1	FUSC family protein	-
JNN61_RS04405	899333..899806	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JNN61_RS04410	899925..901091	-	1167	WP_001563931.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13883.97 Da
• Isoelectric Point: 7.8522

>T188204 WP_001563930.1 NZ_CP068812:896113-896487 [Escherichia coli]
MKTLPVLPGLAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T188204 NZ_CP090355:2271385-2271567 [Pseudomonas kribbensis]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGATGGTATCTGGTCGCCGTCAAAGGCAGTCACCACCAATA
CAAACATCCGGGCAAGCCAGGGAGGGTGACGATCAAGCATCCGGATTCAGATCTTCCCAAGGGAACGATCAACAGCATCT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT188204 WP_001285585.1 NZ_CP068812:895656-896024 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT188204 NZ_CP090355:2271627-2272028 [Pseudomonas kribbensis]
ATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGTGATTATCCCGGATGTACCCGGGTGCTTCTC
CGCAGGCACTACGGTAGCCGAAGCATTTGACAACACTCAGGAGGCACTGGCCTTGCACTATGAAGGACTGGTCGCTGATG
GCGCACCGTTGCCTCGGGTTCAGGACATTGATGCCCATCTCGAGAATCCGGATTACGCCGGCGGTATCTGGGGTGTAGTC
GAGTTTGATATCACTCCCTACTTCGGCAAGTCGGTGCGCTTCAACGCTACGCTCCCGGAGCAGTTGCTGGAGCGTATTGA
CCAGACTGTGCGACGTGATCAGCGTTACAGCTCTCGCTCCGGATTTCTGGCTGCTGCTGCCTTGCGTGAATTGTCAGCTT
AA

Structures

Toxin

• AlphaFold DB: A0A6C9I6Z5

Antitoxin

• AlphaFold DB: A0A7U9LW60