Detailed information of TA system TA188187

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4699094..4699928
• Replicon: chromosome
• Accession: NZ_CP068811
• Organism: Escherichia coli strain RIVM_C028568

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: JNN42_RS22715
• Protein ID: WP_000854820.1
• Coordinates: 4699551..4699928 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: JNN42_RS22710
• Protein ID: WP_001285610.1
• Coordinates: 4699094..4699462 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN42_RS22670	4694815..4695495	+	681	WP_021515201.1	WYL domain-containing protein	-
JNN42_RS22675	4695646..4696323	+	678	WP_060579144.1	hypothetical protein	-
JNN42_RS22680	4696329..4696562	+	234	WP_000883181.1	DUF905 family protein	-
JNN42_RS22685	4696652..4697470	+	819	WP_001175155.1	DUF945 domain-containing protein	-
JNN42_RS22690	4697496..4697636	-	141	WP_000997937.1	hypothetical protein	-
JNN42_RS22695	4697736..4698215	+	480	WP_000706978.1	antirestriction protein	-
JNN42_RS22700	4698230..4698706	+	477	WP_001186193.1	RadC family protein	-
JNN42_RS22705	4698793..4699014	+	222	WP_000692347.1	DUF987 domain-containing protein	-
JNN42_RS22710	4699094..4699462	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN42_RS22715	4699551..4699928	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN42_RS22720	4699925..4700122	+	198	Protein_4447	hypothetical protein	-
JNN42_RS22725	4700150..4700347	+	198	WP_085949158.1	DUF957 domain-containing protein	-
JNN42_RS22730	4700432..4700992	+	561	Protein_4449	DUF4942 domain-containing protein	-
JNN42_RS22735	4701827..4703365	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T188187 WP_000854820.1 NZ_CP068811:4699551-4699928 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T188187 NZ_CP090344:44038-44508 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT188187 WP_001285610.1 NZ_CP068811:4699094-4699462 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188187 NZ_CP090344:43598-44041 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6