Detailed information of TA system TA188176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2565253..2566841
• Replicon: chromosome
• Accession: NZ_CP068811
• Organism: Escherichia coli strain RIVM_C028568

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: JNN42_RS12395
• Protein ID: WP_001551128.1
• Coordinates: 2565519..2566841 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN42_RS12390
• Protein ID: WP_001296726.1
• Coordinates: 2565253..2565519 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN42_RS12390	2565253..2565519	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN42_RS12395	2565519..2566841	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN42_RS12400	2567041..2568393	-	1353	WP_001551127.1	SIR2 family protein	-
JNN42_RS12405	2568608..2569640	-	1033	Protein_2425	ISNCY family transposase	-
JNN42_RS12410	2570007..2570906	-	900	WP_001551125.1	LysR family transcriptional regulator	-
JNN42_RS12415	2571045..2571353	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2568608..2569426	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T188176 WP_001551128.1 NZ_CP068811:2565519-2566841 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T188176 NZ_CP090343:743832-744317 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT188176 WP_001296726.1 NZ_CP068811:2565253-2565519 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT188176 NZ_CP090343:743542-743835 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A829LC11