Detailed information of TA system TA188162

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 881013..881845
• Replicon: chromosome
• Accession: NZ_CP068811
• Organism: Escherichia coli strain RIVM_C028568

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3P5UBZ5
• Locus tag: JNN42_RS04195
• Protein ID: WP_001094454.1
• Coordinates: 881013..881387 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3G8RJV8
• Locus tag: JNN42_RS04200
• Protein ID: WP_001320394.1
• Coordinates: 881477..881845 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN42_RS04165	876126..877274	-	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-
JNN42_RS04170	877346..878329	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
JNN42_RS04175	879140..879310	-	171	Protein_819	IS110 family transposase	-
JNN42_RS04180	879653..880222	-	570	WP_001290250.1	DUF4942 domain-containing protein	-
JNN42_RS04185	880319..880516	-	198	WP_000839276.1	DUF957 domain-containing protein	-
JNN42_RS04190	880528..881016	-	489	WP_000777545.1	hypothetical protein	-
JNN42_RS04195	881013..881387	-	375	WP_001094454.1	TA system toxin CbtA family protein	Toxin
JNN42_RS04200	881477..881845	-	369	WP_001320394.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN42_RS04205	881895..882538	-	644	Protein_825	antitoxin of toxin-antitoxin stability system	-
JNN42_RS04210	882557..882778	-	222	WP_000692300.1	DUF987 domain-containing protein	-
JNN42_RS04215	882841..883317	-	477	WP_001186726.1	RadC family protein	-
JNN42_RS04220	883333..883818	-	486	WP_029701480.1	antirestriction protein	-
JNN42_RS04225	883873..884691	-	819	WP_001234642.1	DUF945 domain-containing protein	-
JNN42_RS04230	884791..885024	-	234	WP_001119719.1	DUF905 family protein	-
JNN42_RS04235	885103..885558	-	456	WP_000581493.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14059.10 Da
• Isoelectric Point: 9.2447

>T188162 WP_001094454.1 NZ_CP068811:c881387-881013 [Escherichia coli]
MNTLPDTHVRKASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T188162 NZ_CP090316:c534924-534628 [Xylella fastidiosa subsp. morus]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCACGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGGGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATCCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTATGGAAGGATTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13623.22 Da
• Isoelectric Point: 6.4783

>AT188162 WP_001320394.1 NZ_CP068811:c881845-881477 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPSTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADANHLDQAFPLLMKQLELMFTSS
ELNPHRQNTVTLYAKGLTCHADTLGSCGYVYLAVYPTPETKQ

Nucleotide

• Length: 369 bp

>AT188162 NZ_CP090316:c534624-534319 [Xylella fastidiosa subsp. morus]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAACTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTATCGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A3P5UBZ5

Antitoxin

• AlphaFold DB: A0A3G8RJV8