Detailed information of TA system TA188154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4564676..4565496
• Replicon: chromosome
• Accession: NZ_CP068810
• Organism: Escherichia coli strain RIVM_C028536

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: A0A0V9MKL8
• Locus tag: JNN60_RS22050
• Protein ID: WP_001550554.1
• Coordinates: 4564676..4564933 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: JNN60_RS22055
• Protein ID: WP_001519303.1
• Coordinates: 4564945..4565496 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN60_RS22030	4559962..4561068	+	1107	WP_001519308.1	N-acetylneuraminate epimerase	-
JNN60_RS22035	4561133..4562113	+	981	WP_001519307.1	transposase	-
JNN60_RS22040	4562696..4563937	-	1242	Protein_4319	helicase YjhR	-
JNN60_RS22045	4564000..4564299	+	300	WP_001519304.1	GNAT family N-acetyltransferase	-
JNN60_RS22050	4564676..4564933	+	258	WP_001550554.1	hypothetical protein	Toxin
JNN60_RS22055	4564945..4565496	+	552	WP_001519303.1	N-acetyltransferase	Antitoxin
JNN60_RS22060	4565548..4565709	+	162	WP_001519302.1	hypothetical protein	-
JNN60_RS22065	4565746..4566294	+	549	WP_001519301.1	hypothetical protein	-
JNN60_RS22070	4566421..4566681	+	261	WP_000084876.1	hypothetical protein	-
JNN60_RS22075	4566719..4566835	+	117	Protein_4326	VOC family protein	-
JNN60_RS22080	4567080..4568201	+	1122	WP_000010833.1	M42 family metallopeptidase	-
JNN60_RS22085	4568198..4568476	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JNN60_RS22090	4568488..4569801	+	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4557160..4565709	8549

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9744.33 Da
• Isoelectric Point: 11.0090

>T188154 WP_001550554.1 NZ_CP068810:4564676-4564933 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKPVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T188154 NZ_CP090316:c480023-479715 [Xylella fastidiosa subsp. morus]
GTGACCATCACAAAGAAAATCAATGTCTCCGAACTGCCAGAGTTCGATGCCGCCGAATACCTGAACAGCGAAGAGGAGGT
AGCCGCGTATCTCACGGCTGTTCTTGAAGAAAACGATCCAGCACTGTTAGCGGCTGCGCTGGGTGACATTGCCCGTTCAC
GTGGTATGTCTCAGATTGCGAAGGATTCTGGCATCACGCGGGAAGCTCTATACAAGGCTCTTCGGCCTGGCAGTGAACCA
CGCTTCGATACGATAAGCCGCGTCTGTACCGCCCTAGGTATACGTCTAGTAGCGCAACCAATGCATTGA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT188154 WP_001519303.1 NZ_CP068810:4564945-4565496 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT188154 NZ_CP090316:c480326-480027 [Xylella fastidiosa subsp. morus]
ATGACCTACACGGTAAAACGACTTGAAGGGTTCTCTGACTGGCTAAAGGGCCTAAAGGATGGGCTGGCAAGGCAGCGCCT
CATCAAGCGCTTACGCAAGGTGCAGCTCGGCAACTTTGGAGACGTGCAGCCAGTGGGTGAAGGCGTGTTTGAAATGCGCG
AACACTTCGGACCTGGCTGGCGTATGTACTACGTGCAGCGAGGCAGCTTTCTCATCGTGATGCTCGGCGGCGGCGACAAG
TCAACGCAGCAATCAGACATTCGTCGGGCCATTGAGCTAGCAAAATCTTTAGAGGATTGA

Structures

Toxin

• AlphaFold DB: A0A0V9MKL8