188150

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3882438..3883056 Replicon chromosome
Accession NZ_CP068810
Organism Escherichia coli strain RIVM_C028536

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN60_RS18700 Protein ID WP_001291435.1
Coordinates 3882838..3883056 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN60_RS18695 Protein ID WP_000344800.1
Coordinates 3882438..3882812 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN60_RS18685 3877527..3878720 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JNN60_RS18690 3878743..3881892 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JNN60_RS18695 3882438..3882812 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN60_RS18700 3882838..3883056 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN60_RS18705 3883228..3883779 + 552 WP_000102564.1 maltose O-acetyltransferase -
JNN60_RS18710 3883895..3884365 + 471 WP_000136192.1 YlaC family protein -
JNN60_RS18715 3884529..3886079 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN60_RS18720 3886121..3886474 - 354 WP_001550741.1 DUF1428 family protein -
JNN60_RS18730 3886853..3887164 + 312 WP_000409911.1 MGMT family protein -
JNN60_RS18735 3887195..3887767 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188150 WP_001291435.1 NZ_CP068810:3882838-3883056 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T188150 NZ_CP090311:c2431227-2430952 [Pseudomonas kielensis]
ATGGAATTGAAGTGGACAAGCAAGGCACTGTCCGACATTGCCAGGCTGTATGAGTTTCTGGCTGCGGTGAATCAGTCTGC
GGCCGCACGCACGGTACAGCAACTTACGGCTGCGCCGATCACGCTGCTGGAAAATCCGCGTATTGGCGAACGCCTGGAAG
AATTTGAGCCTCGGGATGTGCGCCGAATTCAGATCGGCCATTATGAGATGCGTTACGAAATAGTGAATGCCACTATCTAT
CTGCTGCGCCTGTGGCACACCCGTGAGGATCGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT188150 WP_000344800.1 NZ_CP068810:3882438-3882812 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT188150 NZ_CP090311:c2431499-2431227 [Pseudomonas kielensis]
ATGGCAACCACCGCGAAAACCCGATCCGTCACGGCCCACGTCCCTGTACAACTCGCCGAAAGGGTCGATTTGATGGCTGA
ACGCCTGGAGCGCTCCAAGAACTGGATCGTCAAACAGGCTCTATCCGCCTGGATCGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGCGAAGCTTTGGCTGACGTTGACGCTGGCCGCGTAATTGACCACCAAGCTGTCCAGGTCTGGGCAGATAGCCTC
AGCACCGACACCCCGTTGCCGGTACCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References