Detailed information of TA system TA188149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3479924..3480629
• Replicon: chromosome
• Accession: NZ_CP068810
• Organism: Escherichia coli strain RIVM_C028536

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN60_RS16935
• Protein ID: WP_000539521.1
• Coordinates: 3479924..3480310 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN60_RS16940
• Protein ID: WP_001280945.1
• Coordinates: 3480300..3480629 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN60_RS16915	3475928..3476554	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN60_RS16920	3476551..3477666	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN60_RS16925	3477777..3478160	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN60_RS16930	3478373..3479698	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN60_RS16935	3479924..3480310	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN60_RS16940	3480300..3480629	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN60_RS16945	3480699..3482027	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN60_RS16950	3482035..3484383	-	2349	WP_001550886.1	EAL domain-containing protein	-
JNN60_RS16955	3484561..3485472	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T188149 WP_000539521.1 NZ_CP068810:3479924-3480310 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T188149 NZ_CP090305:4301768-4301986 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT188149 WP_001280945.1 NZ_CP068810:3480300-3480629 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT188149 NZ_CP090305:4301368-4301742 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6