Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3214578..3215362 Replicon chromosome
Accession NZ_CP068810
Organism Escherichia coli strain RIVM_C028536

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag JNN60_RS15665 Protein ID WP_000613626.1
Coordinates 3214868..3215362 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag JNN60_RS15660 Protein ID WP_001110447.1
Coordinates 3214578..3214871 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN60_RS15650 3209728..3210687 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
JNN60_RS15655 3211260..3214445 + 3186 WP_001550951.1 ribonuclease E -
JNN60_RS15660 3214578..3214871 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
JNN60_RS15665 3214868..3215362 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
JNN60_RS15670 3215457..3216410 - 954 WP_166742443.1 flagellar hook-associated protein FlgL -
JNN60_RS15675 3216422..3218065 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
JNN60_RS15680 3218131..3219072 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
JNN60_RS15685 3219072..3220169 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T188148 WP_000613626.1 NZ_CP068810:3214868-3215362 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T188148 NZ_CP090305:3545001-3545108 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT188148 WP_001110447.1 NZ_CP068810:3214578-3214871 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT188148 NZ_CP090305:c3544954-3544888 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References