Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2833253..2833891 Replicon chromosome
Accession NZ_CP068810
Organism Escherichia coli strain RIVM_C028536

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9DHD1
Locus tag JNN60_RS13790 Protein ID WP_000813795.1
Coordinates 2833715..2833891 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JNN60_RS13785 Protein ID WP_076797675.1
Coordinates 2833253..2833669 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN60_RS13765 2828405..2829346 - 942 WP_001251315.1 ABC transporter permease -
JNN60_RS13770 2829347..2830360 - 1014 WP_001551095.1 ABC transporter ATP-binding protein -
JNN60_RS13775 2830378..2831523 - 1146 WP_001551094.1 ABC transporter substrate-binding protein -
JNN60_RS13780 2831768..2833174 - 1407 WP_001551093.1 PLP-dependent aminotransferase family protein -
JNN60_RS13785 2833253..2833669 - 417 WP_076797675.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JNN60_RS13790 2833715..2833891 - 177 WP_000813795.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JNN60_RS13795 2834113..2834343 + 231 WP_023910283.1 YncJ family protein -
JNN60_RS13800 2834435..2836396 - 1962 WP_001551090.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JNN60_RS13805 2836469..2837005 - 537 WP_001551089.1 DNA-binding transcriptional regulator SutR -
JNN60_RS13810 2837058..2838269 + 1212 WP_071591517.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-65)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6747.80 Da        Isoelectric Point: 11.5666

>T188147 WP_000813795.1 NZ_CP068810:c2833891-2833715 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T188147 NZ_CP090305:3544466-3544573 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15220.55 Da        Isoelectric Point: 4.7386

>AT188147 WP_076797675.1 NZ_CP068810:c2833669-2833253 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT188147 NZ_CP090305:c3544419-3544353 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DHD1

Antitoxin

Source ID Structure

References