Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1045274..1045928 Replicon chromosome
Accession NZ_CP068810
Organism Escherichia coli strain RIVM_C028536

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JNN60_RS05050 Protein ID WP_000244781.1
Coordinates 1045521..1045928 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag JNN60_RS05045 Protein ID WP_001564007.1
Coordinates 1045274..1045540 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN60_RS05025 1041362..1042795 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
JNN60_RS05030 1042840..1043151 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
JNN60_RS05035 1043315..1043974 + 660 WP_000250274.1 hemolysin III family protein -
JNN60_RS05040 1044051..1045031 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
JNN60_RS05045 1045274..1045540 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
JNN60_RS05050 1045521..1045928 + 408 WP_000244781.1 protein YgfX Toxin
JNN60_RS05055 1045968..1046489 - 522 WP_001055874.1 flavodoxin FldB -
JNN60_RS05060 1046601..1047497 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JNN60_RS05065 1047522..1048232 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JNN60_RS05070 1048238..1049971 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T188135 WP_000244781.1 NZ_CP068810:1045521-1045928 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T188135 NZ_CP090305:1171177-1171284 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTTGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT188135 WP_001564007.1 NZ_CP068810:1045274-1045540 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT188135 NZ_CP090305:c1171129-1171063 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References