Detailed information of TA system TA188134

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 904301..905099
• Replicon: chromosome
• Accession: NZ_CP068810
• Organism: Escherichia coli strain RIVM_C028536

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: JNN60_RS04320
• Protein ID: WP_000854735.1
• Coordinates: 904301..904678 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: JNN60_RS04325
• Protein ID: WP_001285415.1
• Coordinates: 904725..905099 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN60_RS04290	900269..901534	-	1266	WP_001218869.1	integrase arm-type DNA-binding domain-containing protein	-
JNN60_RS04295	901998..902324	-	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JNN60_RS04300	902321..902584	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
JNN60_RS04305	902656..903522	-	867	WP_001280433.1	DUF4942 domain-containing protein	-
JNN60_RS04310	903607..903804	-	198	WP_000839291.1	DUF957 domain-containing protein	-
JNN60_RS04315	903816..904304	-	489	WP_000761676.1	hypothetical protein	-
JNN60_RS04320	904301..904678	-	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
JNN60_RS04325	904725..905099	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN60_RS04330	905179..905400	-	222	WP_000692309.1	DUF987 domain-containing protein	-
JNN60_RS04335	905463..905939	-	477	WP_001366855.1	RadC family protein	-
JNN60_RS04340	905955..906434	-	480	WP_000844100.1	antirestriction protein	-
JNN60_RS04345	906516..907334	-	819	WP_001234629.1	DUF945 domain-containing protein	-
JNN60_RS04350	907433..907666	-	234	WP_001278287.1	DUF905 family protein	-
JNN60_RS04355	907672..908349	-	678	WP_042104028.1	hypothetical protein	-
JNN60_RS04360	908497..909177	-	681	WP_001593697.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX	788631..938676	150045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T188134 WP_000854735.1 NZ_CP068810:c904678-904301 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T188134 NZ_CP090305:c529965-529792 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT188134 WP_001285415.1 NZ_CP068810:c905099-904725 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT188134 NZ_CP090305:c530289-529993 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A067H947