Detailed information of TA system TA188130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 161180..161792
• Replicon: chromosome
• Accession: NZ_CP068810
• Organism: Escherichia coli strain RIVM_C028536

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JNN60_RS00685
• Protein ID: WP_000833473.1
• Coordinates: 161180..161365 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JNN60_RS00690
• Protein ID: WP_000499744.1
• Coordinates: 161382..161792 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN60_RS00675	158021..159172	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
JNN60_RS00680	159373..160911	+	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
JNN60_RS00685	161180..161365	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JNN60_RS00690	161382..161792	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JNN60_RS00695	161864..163828	-	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
JNN60_RS00700	163839..165239	-	1401	WP_000204811.1	MFS transporter	-
JNN60_RS00705	165465..166280	+	816	WP_000891823.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T188130 WP_000833473.1 NZ_CP068810:161180-161365 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T188130 NZ_CP090304:4562866-4563357 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT188130 WP_000499744.1 NZ_CP068810:161382-161792 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT188130 NZ_CP090304:4562576-4562869 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9