Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3652168..3652786 Replicon chromosome
Accession NZ_CP068809
Organism Escherichia coli strain RIVM_C028497

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN39_RS17435 Protein ID WP_001291435.1
Coordinates 3652568..3652786 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN39_RS17430 Protein ID WP_000344800.1
Coordinates 3652168..3652542 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN39_RS17420 3647258..3648451 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JNN39_RS17425 3648474..3651623 + 3150 WP_001132481.1 multidrug efflux RND transporter permease subunit -
JNN39_RS17430 3652168..3652542 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN39_RS17435 3652568..3652786 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN39_RS17440 3652960..3653511 + 552 WP_000102539.1 maltose O-acetyltransferase -
JNN39_RS17445 3653627..3654097 + 471 WP_000136192.1 YlaC family protein -
JNN39_RS17450 3654261..3655811 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN39_RS17455 3655853..3656206 - 354 WP_000878138.1 DUF1428 family protein -
JNN39_RS17465 3656585..3656896 + 312 WP_000409908.1 MGMT family protein -
JNN39_RS17470 3656927..3657499 - 573 WP_000779830.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T188117 WP_001291435.1 NZ_CP068809:3652568-3652786 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T188117 NZ_CP090245:c5433099-5432842 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGGCTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT188117 WP_000344800.1 NZ_CP068809:3652168-3652542 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT188117 NZ_CP090245:c5432831-5432280 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGATTTGCTCAACGATGAAAGTGCTCACCCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCAGTTGGGCATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCGCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCGCTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGGGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References