Detailed information of TA system TA188115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3160666..3162386
• Replicon: chromosome
• Accession: NZ_CP068809
• Organism: Escherichia coli strain RIVM_C028497

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A7U9J2A7
• Locus tag: JNN39_RS15070
• Protein ID: WP_000312833.1
• Coordinates: 3160666..3161322 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: B7UP19
• Locus tag: JNN39_RS15075
• Protein ID: WP_000634203.1
• Coordinates: 3161319..3162386 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN39_RS15050	3156066..3156320	-	255	WP_154670580.1	hypothetical protein	-
JNN39_RS15055	3156602..3156841	+	240	WP_001431807.1	hypothetical protein	-
JNN39_RS15060	3156881..3159319	+	2439	WP_000985465.1	type I restriction-modification system subunit M	-
JNN39_RS15065	3159319..3160653	+	1335	WP_000228013.1	restriction endonuclease subunit S	-
JNN39_RS15070	3160666..3161322	+	657	WP_000312833.1	DUF4433 domain-containing protein	Toxin
JNN39_RS15075	3161319..3162386	+	1068	WP_000634203.1	macro domain-containing protein	Antitoxin
JNN39_RS15080	3162405..3165500	+	3096	WP_000108735.1	HsdR family type I site-specific deoxyribonuclease	-
JNN39_RS15085	3165500..3166216	+	717	WP_001122107.1	M48 family metallopeptidase	-
JNN39_RS15090	3166561..3167175	+	615	Protein_2962	IS3-like element IS600 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	aafC / aggD / iroC / iroD / iroE / iroN	3154659..3191742	37083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25233.56 Da
• Isoelectric Point: 6.2145

>T188115 WP_000312833.1 NZ_CP068809:3160666-3161322 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T188115 NZ_CP090245:4401130-4401498 [Klebsiella oxytoca]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACAACACACCGTTTTGCAACGACAGTGTGATCCAGAAGCACATTGATGCGG
GTATCTCACTGTGTGACGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGCAAGGCTACCGGGCTGATGCCCCGCAAGGGCTA
TCAAACCGTTACTGACATTACCAGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40672.70 Da
• Isoelectric Point: 5.6927

>AT188115 WP_000634203.1 NZ_CP068809:3161319-3162386 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT188115 NZ_CP090245:4400715-4401074 [Klebsiella oxytoca]
ATGCAGAAAGCAACCCGTGTAATCAACCATAATATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
TGGTGCCCGGCTTGTACAGGAGGGTAACCACCTGCATTACCTAGCTGACCGCGCCAGCATTGCTGGGACATTCAGCGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTAGTGATGAAGCAGATGGAGCTCATGCTGACCAGCAACGAACTCACG
CCCCACATTCAGCGCTGCATCACGTTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
TTACATCGTAATTTATCCGACATCTGCAACAACCGCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9J2A7

Antitoxin

• AlphaFold DB: A0A7U9J2B9