Detailed information of TA system TA188114

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3143044..3143888
• Replicon: chromosome
• Accession: NZ_CP068809
• Organism: Escherichia coli strain RIVM_C028497

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: JNN39_RS14985
• Protein ID: WP_000854686.1
• Coordinates: 3143044..3143427 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: JNN39_RS14990
• Protein ID: WP_001285602.1
• Coordinates: 3143508..3143888 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN39_RS14955	3139720..3140424	+	705	WP_001241673.1	leucyl/phenylalanyl-tRNA--protein transferase	-
JNN39_RS14960	3140709..3140927	+	219	WP_001040187.1	translation initiation factor IF-1	-
JNN39_RS14970	3141418..3142260	-	843	WP_001431817.1	DUF4942 domain-containing protein	-
JNN39_RS14975	3142345..3142542	-	198	WP_000839253.1	DUF957 domain-containing protein	-
JNN39_RS14980	3142559..3143047	-	489	WP_001054233.1	hypothetical protein	-
JNN39_RS14985	3143044..3143427	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
JNN39_RS14990	3143508..3143888	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN39_RS14995	3143899..3144582	-	684	WP_000086768.1	hypothetical protein	-
JNN39_RS15000	3144601..3144822	-	222	WP_000692298.1	DUF987 domain-containing protein	-
JNN39_RS15005	3144885..3145361	-	477	WP_001186726.1	RadC family protein	-
JNN39_RS15010	3145377..3145862	-	486	WP_000214307.1	antirestriction protein	-
JNN39_RS15015	3145954..3146772	-	819	WP_001234732.1	DUF945 domain-containing protein	-
JNN39_RS15020	3146872..3147105	-	234	WP_001119719.1	DUF905 family protein	-
JNN39_RS15025	3147184..3147639	-	456	WP_001504120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T188114 WP_000854686.1 NZ_CP068809:c3143427-3143044 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T188114 NZ_CP090245:2992279-2992382 [Klebsiella oxytoca]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT188114 WP_001285602.1 NZ_CP068809:c3143888-3143508 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT188114 NZ_CP090245:c2992389-2992244 [Klebsiella oxytoca]
AAAAGATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGT