Detailed information of TA system TA188093

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4784238..4785072
• Replicon: chromosome
• Accession: NZ_CP068808
• Organism: Escherichia coli strain RIVM_C018699

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: JNN47_RS23310
• Protein ID: WP_000854820.1
• Coordinates: 4784695..4785072 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: JNN47_RS23305
• Protein ID: WP_001285610.1
• Coordinates: 4784238..4784606 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN47_RS23270	4779959..4780639	+	681	WP_021515201.1	WYL domain-containing protein	-
JNN47_RS23275	4780790..4781467	+	678	WP_060579144.1	hypothetical protein	-
JNN47_RS23280	4781473..4781706	+	234	WP_000883181.1	DUF905 family protein	-
JNN47_RS23285	4781796..4782614	+	819	WP_001175155.1	DUF945 domain-containing protein	-
JNN47_RS23290	4782880..4783359	+	480	WP_000706978.1	antirestriction protein	-
JNN47_RS23295	4783374..4783850	+	477	WP_001186193.1	RadC family protein	-
JNN47_RS23300	4783937..4784158	+	222	WP_000692347.1	DUF987 domain-containing protein	-
JNN47_RS23305	4784238..4784606	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN47_RS23310	4784695..4785072	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN47_RS23315	4785069..4785266	+	198	Protein_4565	hypothetical protein	-
JNN47_RS23320	4785294..4785491	+	198	WP_085949158.1	DUF957 domain-containing protein	-
JNN47_RS23325	4785576..4786136	+	561	Protein_4567	DUF4942 domain-containing protein	-
JNN47_RS23330	4786971..4788509	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4750948..4796322	45374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T188093 WP_000854820.1 NZ_CP068808:4784695-4785072 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T188093 NZ_CP090242:c3207764-3207480 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT188093 WP_001285610.1 NZ_CP068808:4784238-4784606 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT188093 NZ_CP090242:c3207996-3207754 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6