Detailed information of TA system TA188083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2735562..2736200
• Replicon: chromosome
• Accession: NZ_CP068808
• Organism: Escherichia coli strain RIVM_C018699

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN47_RS13295
• Protein ID: WP_000813795.1
• Coordinates: 2736024..2736200 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN47_RS13290
• Protein ID: WP_076797675.1
• Coordinates: 2735562..2735978 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN47_RS13270	2730714..2731655	-	942	WP_001251315.1	ABC transporter permease	-
JNN47_RS13275	2731656..2732669	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN47_RS13280	2732687..2733832	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN47_RS13285	2734077..2735483	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN47_RS13290	2735562..2735978	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN47_RS13295	2736024..2736200	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN47_RS13300	2736422..2736652	+	231	WP_023910283.1	YncJ family protein	-
JNN47_RS13305	2736744..2738705	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN47_RS13310	2738778..2739314	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN47_RS13315	2739367..2740578	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T188083 WP_000813795.1 NZ_CP068808:c2736200-2736024 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T188083 NZ_CP090222:3821568-3821786 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT188083 WP_076797675.1 NZ_CP068808:c2735978-2735562 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT188083 NZ_CP090222:3821167-3821541 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1