Detailed information of TA system TA188050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2749141..2749753
• Replicon: chromosome
• Accession: NZ_CP068807
• Organism: Escherichia coli strain RIVM_C018583

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: JNN51_RS13270
• Protein ID: WP_000833473.1
• Coordinates: 2749568..2749753 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: JNN51_RS13265
• Protein ID: WP_000499744.1
• Coordinates: 2749141..2749551 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN51_RS13250	2744653..2745468	-	816	WP_000891823.1	AraC family transcriptional regulator	-
JNN51_RS13255	2745694..2747094	+	1401	WP_000204811.1	MFS transporter	-
JNN51_RS13260	2747105..2749069	+	1965	WP_001551835.1	glycoside hydrolase family 127 protein	-
JNN51_RS13265	2749141..2749551	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JNN51_RS13270	2749568..2749753	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
JNN51_RS13275	2750022..2751560	-	1539	WP_001551836.1	aldehyde dehydrogenase AldB	-
JNN51_RS13280	2751761..2752912	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T188050 WP_000833473.1 NZ_CP068807:c2749753-2749568 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T188050 NZ_CP090212:1020437-1020772 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT188050 WP_000499744.1 NZ_CP068807:c2749551-2749141 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT188050 NZ_CP090212:1020189-1020437 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9