Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1906174..1906828 Replicon chromosome
Accession NZ_CP068807
Organism Escherichia coli strain RIVM_C018583

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JNN51_RS09155 Protein ID WP_000244781.1
Coordinates 1906174..1906581 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag JNN51_RS09160 Protein ID WP_001564007.1
Coordinates 1906562..1906828 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN51_RS09135 1902131..1903864 - 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -
JNN51_RS09140 1903870..1904580 - 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JNN51_RS09145 1904605..1905501 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JNN51_RS09150 1905613..1906134 + 522 WP_001055874.1 flavodoxin FldB -
JNN51_RS09155 1906174..1906581 - 408 WP_000244781.1 protein YgfX Toxin
JNN51_RS09160 1906562..1906828 - 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
JNN51_RS09165 1907071..1908051 + 981 WP_001564008.1 tRNA-modifying protein YgfZ -
JNN51_RS09170 1908128..1908787 - 660 WP_000250274.1 hemolysin III family protein -
JNN51_RS09175 1908951..1909262 - 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
JNN51_RS09180 1909307..1910740 + 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T188047 WP_000244781.1 NZ_CP068807:c1906581-1906174 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T188047 NZ_CP090212:699045-699359 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT188047 WP_001564007.1 NZ_CP068807:c1906828-1906562 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT188047 NZ_CP090212:699356-699772 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References