Detailed information of TA system TA187994

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4705074..4705906
• Replicon: chromosome
• Accession: NZ_CP068805
• Organism: Escherichia coli strain RIVM_C018567

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3P5UBZ5
• Locus tag: JNN55_RS22885
• Protein ID: WP_001094454.1
• Coordinates: 4705532..4705906 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3G8RJV8
• Locus tag: JNN55_RS22880
• Protein ID: WP_001320394.1
• Coordinates: 4705074..4705442 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN55_RS22845	4701361..4701816	+	456	WP_000581493.1	hypothetical protein	-
JNN55_RS22850	4701895..4702128	+	234	WP_001119729.1	DUF905 family protein	-
JNN55_RS22855	4702228..4703046	+	819	WP_072855048.1	DUF945 domain-containing protein	-
JNN55_RS22860	4703101..4703586	+	486	WP_029701480.1	antirestriction protein	-
JNN55_RS22865	4703602..4704078	+	477	WP_001186726.1	RadC family protein	-
JNN55_RS22870	4704141..4704362	+	222	WP_000692300.1	DUF987 domain-containing protein	-
JNN55_RS22875	4704381..4705024	+	644	Protein_4480	antitoxin of toxin-antitoxin stability system	-
JNN55_RS22880	4705074..4705442	+	369	WP_001320394.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN55_RS22885	4705532..4705906	+	375	WP_001094454.1	TA system toxin CbtA family protein	Toxin
JNN55_RS22890	4705903..4706391	+	489	WP_000777545.1	hypothetical protein	-
JNN55_RS22895	4706403..4706600	+	198	WP_000839276.1	DUF957 domain-containing protein	-
JNN55_RS22900	4706697..4707266	+	570	WP_001290250.1	DUF4942 domain-containing protein	-
JNN55_RS22905	4708015..4709553	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14059.10 Da
• Isoelectric Point: 9.2447

>T187994 WP_001094454.1 NZ_CP068805:4705532-4705906 [Escherichia coli]
MNTLPDTHVRKASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T187994 NZ_CP090196:c628144-627680 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13623.22 Da
• Isoelectric Point: 6.4783

>AT187994 WP_001320394.1 NZ_CP068805:4705074-4705442 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPSTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADANHLDQAFPLLMKQLELMFTSS
ELNPHRQNTVTLYAKGLTCHADTLGSCGYVYLAVYPTPETKQ

Nucleotide

• Length: 369 bp

>AT187994 NZ_CP090196:c628479-628144 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3P5UBZ5

Antitoxin

• AlphaFold DB: A0A3G8RJV8