Detailed information of TA system TA187985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3142890..3143674
• Replicon: chromosome
• Accession: NZ_CP068805
• Organism: Escherichia coli strain RIVM_C018567

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JNN55_RS15360
• Protein ID: WP_000613626.1
• Coordinates: 3143180..3143674 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JNN55_RS15355
• Protein ID: WP_001110447.1
• Coordinates: 3142890..3143183 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN55_RS15345	3138040..3138999	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JNN55_RS15350	3139572..3142757	+	3186	WP_001550951.1	ribonuclease E	-
JNN55_RS15355	3142890..3143183	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JNN55_RS15360	3143180..3143674	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JNN55_RS15365	3143769..3144722	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
JNN55_RS15370	3144734..3146377	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
JNN55_RS15375	3146443..3147384	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JNN55_RS15380	3147384..3148481	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T187985 WP_000613626.1 NZ_CP068805:3143180-3143674 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T187985 NZ_CP090192:c2293788-2293303 [Enterobacter hormaechei]
GTGGGACGTGTAACCCCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCATCAGTGGAGAGGCCGTGCTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAATGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT187985 WP_001110447.1 NZ_CP068805:3142890-3143183 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT187985 NZ_CP090192:c2294042-2293776 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6