Detailed information of TA system TA187974

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2001800..2002631
• Replicon: chromosome
• Accession: NZ_CP068805
• Organism: Escherichia coli strain RIVM_C018567

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: JNN55_RS09610
• Protein ID: WP_000854814.1
• Coordinates: 2001800..2002174 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: JNN55_RS09615
• Protein ID: WP_001285585.1
• Coordinates: 2002263..2002631 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN55_RS09570	1997196..1998362	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
JNN55_RS09575	1998481..1998954	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
JNN55_RS09580	1999152..2000210	+	1059	WP_001200891.1	FUSC family protein	-
JNN55_RS09585	2000382..2000711	+	330	WP_000450409.1	DUF496 family protein	-
JNN55_RS09590	2000812..2000994	-	183	WP_001016348.1	hypothetical protein	-
JNN55_RS09595	2001066..2001194	+	129	Protein_1879	transposase domain-containing protein	-
JNN55_RS09600	2001483..2001596	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN55_RS09605	2001609..2001803	-	195	WP_000988600.1	hypothetical protein	-
JNN55_RS09610	2001800..2002174	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
JNN55_RS09615	2002263..2002631	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
JNN55_RS09620	2002705..2002926	-	222	WP_000692323.1	DUF987 domain-containing protein	-
JNN55_RS09625	2002989..2003465	-	477	WP_001186726.1	RadC family protein	-
JNN55_RS09630	2003481..2003960	-	480	WP_134259499.1	antirestriction protein	-
JNN55_RS09635	2004042..2004860	-	819	WP_001234629.1	DUF945 domain-containing protein	-
JNN55_RS09640	2004960..2005193	-	234	WP_001119729.1	DUF905 family protein	-
JNN55_RS09645	2005272..2005727	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T187974 WP_000854814.1 NZ_CP068805:c2002174-2001800 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T187974 NZ_CP090179:569234-569566 [Vibrio harveyi]
ATGAAAAGTGTATTTGTCGAATCAACCATATTTGAAAAGTACCGTAATGAATATCTCAGCGATGATGAGTTTAGGCTTTT
TCAAGCAGAGTTAATGTCTAACCCGAAGCAGGGTGACGTGATTCAAGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAGCGTGGTGGCTCTCGCGTTATCTATTACTTCCTCGATGAAAAGCGTCGTTTCTACTTGCTGACG
ATTTACGGCAAGAATGAAATGTCCGACTTAACCGTAGACCAAAAGAAACAACTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT187974 WP_001285585.1 NZ_CP068805:c2002631-2002263 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT187974 NZ_CP090179:569553-569867 [Vibrio harveyi]
ATGAGCAATCGTAATTTATTTGCGGAGTTAAGTTCAGCACTTGTTGAGGCTAAAGAGCATTCAGAGGGCAAGCTTACCCT
TAAAACTCATCAAGTTAACGACATCAGTGAGTTAAATATCTCACCTGATGAAATCGTGAGTATTCGAGAGCAGTTTAATA
TGTCTCGTGGTGTGTTTGCTCGTTTGTTGCACACATCATCTCGTACGTTAGAAAACTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCAGTAACTCTTCTAAAGTTGGTTCAGCGTCATCCAGAAACCCTGTCGCACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60