Detailed information of TA system TA187961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4409549..4410057
• Replicon: chromosome
• Accession: NZ_CP068804
• Organism: Escherichia coli strain RIVM_C018563

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JNN37_RS21195
• Protein ID: WP_001534843.1
• Coordinates: 4409848..4410057 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: JNN37_RS21190
• Protein ID: WP_000063161.1
• Coordinates: 4409549..4409851 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN37_RS21175	4406077..4408227	+	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-
JNN37_RS21180	4408345..4408548	+	204	WP_001534846.1	YbdD/YjiX family protein	-
JNN37_RS21185	4408559..4409515	+	957	WP_001534845.1	GTPase	-
JNN37_RS21190	4409549..4409851	-	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
JNN37_RS21195	4409848..4410057	-	210	WP_001534843.1	BrnT family toxin	Toxin
JNN37_RS21200	4410153..4411319	+	1167	WP_001534841.1	restriction endonuclease	-
JNN37_RS21205	4411383..4413002	+	1620	WP_001550561.1	type I restriction-modification system subunit M	-
JNN37_RS21210	4412992..4414302	+	1311	WP_001534837.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T187961 WP_001534843.1 NZ_CP068804:c4410057-4409848 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T187961 NZ_CP090146:c1098251-1098033 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT187961 WP_000063161.1 NZ_CP068804:c4409851-4409549 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT187961 NZ_CP090146:c1098654-1098280 [Enterobacter hormaechei]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3