Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3801043..3801661 Replicon chromosome
Accession NZ_CP068804
Organism Escherichia coli strain RIVM_C018563

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JNN37_RS18240 Protein ID WP_001291435.1
Coordinates 3801443..3801661 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JNN37_RS18235 Protein ID WP_000344800.1
Coordinates 3801043..3801417 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN37_RS18225 3796132..3797325 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JNN37_RS18230 3797348..3800497 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JNN37_RS18235 3801043..3801417 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JNN37_RS18240 3801443..3801661 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JNN37_RS18245 3801833..3802384 + 552 WP_000102564.1 maltose O-acetyltransferase -
JNN37_RS18250 3802500..3802970 + 471 WP_000136192.1 YlaC family protein -
JNN37_RS18255 3803134..3804684 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JNN37_RS18260 3804726..3805079 - 354 WP_001550741.1 DUF1428 family protein -
JNN37_RS18270 3805458..3805769 + 312 WP_000409911.1 MGMT family protein -
JNN37_RS18275 3805800..3806372 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T187958 WP_001291435.1 NZ_CP068804:3801443-3801661 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T187958 NZ_CP090141:4454776-4455087 [Salmonella enterica subsp. enterica serovar Goldcoast]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGATAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT187958 WP_000344800.1 NZ_CP068804:3801043-3801417 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT187958 NZ_CP090141:4455084-4455530 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References