Detailed information of TA system TA187955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2751936..2752574
• Replicon: chromosome
• Accession: NZ_CP068804
• Organism: Escherichia coli strain RIVM_C018563

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN37_RS13340
• Protein ID: WP_000813795.1
• Coordinates: 2752398..2752574 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN37_RS13335
• Protein ID: WP_076797675.1
• Coordinates: 2751936..2752352 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN37_RS13315	2747088..2748029	-	942	WP_001251315.1	ABC transporter permease	-
JNN37_RS13320	2748030..2749043	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN37_RS13325	2749061..2750206	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN37_RS13330	2750451..2751857	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN37_RS13335	2751936..2752352	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN37_RS13340	2752398..2752574	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN37_RS13345	2752796..2753026	+	231	WP_023910283.1	YncJ family protein	-
JNN37_RS13350	2753118..2755079	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN37_RS13355	2755152..2755688	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN37_RS13360	2755741..2756952	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T187955 WP_000813795.1 NZ_CP068804:c2752574-2752398 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T187955 NZ_CP090141:3498509-3498754 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCGGACGGGTGTATTGTGCTAATGG
CGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTATCAGGCTGAACAGGTAGTTAAGGGGATGTGGGACGTGATT
GTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT187955 WP_076797675.1 NZ_CP068804:c2752352-2751936 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT187955 NZ_CP090141:c3498718-3498362 [Salmonella enterica subsp. enterica serovar Goldcoast]
TTCAGCCTGATAAAGCTGTTCGCACAGCTTCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCGTCCGAAATCT
TCACCGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGGCAGTCGCCGCTGGGGATTTGTGAGTAACAGGCGGTC
ATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTC
CGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGCGAGTATGGGCGCGACATCCTGGCATCGCGCCTCTCTCTCCCCG
GCCAGTGATGAGCAACCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1