187932

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3525720..3526557 Replicon chromosome
Accession NZ_CP068803
Organism Escherichia coli strain RIVM_C018404

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JNN34_RS17005 Protein ID WP_000227784.1
Coordinates 3526015..3526557 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JNN34_RS17000 Protein ID WP_001297137.1
Coordinates 3525720..3526031 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN34_RS16975 3520740..3521687 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JNN34_RS16980 3521709..3523700 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JNN34_RS16985 3523690..3524304 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JNN34_RS16990 3524304..3524633 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JNN34_RS16995 3524645..3525535 + 891 WP_000971336.1 heme o synthase -
JNN34_RS17000 3525720..3526031 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JNN34_RS17005 3526015..3526557 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JNN34_RS17010 3526613..3527548 - 936 WP_001297127.1 sel1 repeat family protein -
JNN34_RS17015 3527956..3529320 + 1365 WP_001000978.1 MFS transporter -
JNN34_RS17020 3529448..3529939 - 492 WP_001138904.1 nucleotide binding protein YajQ -
JNN34_RS17025 3530107..3531018 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T187932 WP_000227784.1 NZ_CP068803:3526015-3526557 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T187932 NZ_CP090133:3307615-3307833 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT187932 WP_001297137.1 NZ_CP068803:3525720-3526031 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT187932 NZ_CP090133:3307213-3307587 [Salmonella enterica subsp. enterica serovar Goldcoast]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References