Detailed information of TA system TA187921

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1827415..1828250
• Replicon: chromosome
• Accession: NZ_CP068803
• Organism: Escherichia coli strain RIVM_C018404

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: JNN34_RS08690
• Protein ID: WP_000854761.1
• Coordinates: 1827415..1827792 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: JNN34_RS08695
• Protein ID: WP_001295723.1
• Coordinates: 1827882..1828250 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN34_RS08655	1822804..1822986	-	183	WP_001016348.1	hypothetical protein	-
JNN34_RS08660	1823440..1823811	-	372	WP_001295631.1	IS110 family transposase	-
JNN34_RS08665	1824323..1824727	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
JNN34_RS08670	1824724..1825071	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
JNN34_RS08675	1825120..1826655	+	1536	WP_001333339.1	IS66-like element ISEc22 family transposase	-
JNN34_RS08680	1827090..1827203	-	114	WP_001161660.1	DUF957 domain-containing protein	-
JNN34_RS08685	1827216..1827418	-	203	Protein_1696	hypothetical protein	-
JNN34_RS08690	1827415..1827792	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
JNN34_RS08695	1827882..1828250	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN34_RS08700	1828413..1828634	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JNN34_RS08705	1828697..1829173	-	477	WP_001186774.1	RadC family protein	-
JNN34_RS08710	1829189..1829662	-	474	WP_000855059.1	antirestriction protein	-
JNN34_RS08715	1830004..1830789	-	786	WP_114427785.1	DUF945 domain-containing protein	-
JNN34_RS08720	1830970..1832511	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1824323..1833272	8949

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T187921 WP_000854761.1 NZ_CP068803:c1827792-1827415 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T187921 NZ_CP090127:c93904-93620 [Klebsiella pneumoniae]
ATGAAAGAAGAACTTAAAAAATTTGATGTTGTTGAATTTCTTGAAGATGAAGAAGATATTCAGGAATATCTTAATGCGGC
AATTGAAGAAGATGATACAAAATATCTTTTTAAAGCATTAGGTAACATAGCCAGAGCAAGAAACATAAGTCAGCTATCTA
AAGAAGTTGGTATGTCCAGGGAGGGAATTTACAAGGCGTTATCCGGTGAAGGAAATCCATCATTCCAGACTGTCTCTAAA
ATCACAAAAGCACTTGGTTTAAAACTTCACTTCACAGGGGCTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT187921 WP_001295723.1 NZ_CP068803:c1828250-1827882 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT187921 NZ_CP090127:c94202-93906 [Klebsiella pneumoniae]
ATGATAGAAATAAAACGGACGCCGGAAGTTGAAAAGTGGCTCAGGTCTTTGAAGGACAGAACGGCAAAGGCAAAAATATT
GATGCGACTTGAGCGCATGGAGGACGGAAATTTTGGAGACGTGGAGCCAATAGGTGACGGATTATCAGAATTAAGGATTC
ATCAGGGTAAAGGCTACCGTGTGTATTTTGGAAACAAAAACAATCAAATTATTCTCTTGCTTTGTGGTGGAGATAAAAGC
ACGCAGCAAGCAGATATCAGAAAAGCGAAACAATTAGCTAAACAGTGGGGGTTTTAG

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52