Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1059311..1059894 Replicon chromosome
Accession NZ_CP068803
Organism Escherichia coli strain RIVM_C018404

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag JNN34_RS05040 Protein ID WP_000254745.1
Coordinates 1059559..1059894 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3P5DUY8
Locus tag JNN34_RS05035 Protein ID WP_074502498.1
Coordinates 1059311..1059559 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN34_RS05025 1055650..1056951 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JNN34_RS05030 1056999..1059233 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JNN34_RS05035 1059311..1059559 + 249 WP_074502498.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JNN34_RS05040 1059559..1059894 + 336 WP_000254745.1 endoribonuclease MazF Toxin
JNN34_RS05045 1059965..1060756 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JNN34_RS05050 1060984..1062621 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JNN34_RS05055 1062709..1064007 + 1299 WP_000036723.1 phosphopyruvate hydratase -
JNN34_RS05060 1064063..1064425 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T187918 WP_000254745.1 NZ_CP068803:1059559-1059894 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T187918 NZ_CP090126:c4828067-4827783 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9359.72 Da        Isoelectric Point: 4.4791

>AT187918 WP_074502498.1 NZ_CP068803:1059311-1059559 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGESKDKE
VW

Download         Length: 249 bp

>AT187918 NZ_CP090126:c4828299-4828057 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A3P5DUY8

References