Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4114260..4114914 Replicon chromosome
Accession NZ_CP068802
Organism Escherichia coli strain RIVM_C018249

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JNN40_RS19670 Protein ID WP_000244777.1
Coordinates 4114507..4114914 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A7B2N3K9
Locus tag JNN40_RS19665 Protein ID WP_001604807.1
Coordinates 4114260..4114526 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN40_RS19640 4109543..4110286 + 744 WP_001366570.1 SDR family oxidoreductase -
JNN40_RS19645 4110348..4111781 - 1434 WP_001604813.1 6-phospho-beta-glucosidase BglA -
JNN40_RS19650 4111826..4112137 + 312 WP_001604811.1 N(4)-acetylcytidine aminohydrolase -
JNN40_RS19655 4112301..4112960 + 660 WP_000250269.1 hemolysin III family protein -
JNN40_RS19660 4113037..4114017 - 981 WP_001604809.1 tRNA-modifying protein YgfZ -
JNN40_RS19665 4114260..4114526 + 267 WP_001604807.1 FAD assembly factor SdhE Antitoxin
JNN40_RS19670 4114507..4114914 + 408 WP_000244777.1 toxin CptA Toxin
JNN40_RS19675 4114954..4115475 - 522 WP_001055874.1 flavodoxin FldB -
JNN40_RS19680 4115587..4116483 + 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
JNN40_RS19685 4116508..4117218 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JNN40_RS19690 4117224..4118957 + 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T187913 WP_000244777.1 NZ_CP068802:4114507-4114914 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T187913 NZ_CP090126:2004031-2004133 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10595.21 Da        Isoelectric Point: 5.6375

>AT187913 WP_001604807.1 NZ_CP068802:4114260-4114526 [Escherichia coli]
MYINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT187913 NZ_CP090126:c2004140-2003995 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
AlphaFold DB A0A7B2N3K9

References