Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3358089..3358701 Replicon chromosome
Accession NZ_CP068802
Organism Escherichia coli strain RIVM_C018249

Toxin (Protein)


Gene name hicA Uniprot ID A0A167AMN3
Locus tag JNN40_RS16020 Protein ID WP_000833474.1
Coordinates 3358089..3358274 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag JNN40_RS16025 Protein ID WP_000499746.1
Coordinates 3358291..3358701 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN40_RS16005 3353606..3354757 + 1152 WP_000741506.1 L-threonine dehydrogenase -
JNN40_RS16010 3354958..3356496 + 1539 WP_001469350.1 aldehyde dehydrogenase AldB -
JNN40_RS16015 3356537..3357616 - 1080 WP_202859858.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
JNN40_RS16020 3358089..3358274 + 186 WP_000833474.1 type II toxin-antitoxin system HicA family toxin Toxin
JNN40_RS16025 3358291..3358701 + 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JNN40_RS16030 3358773..3360737 - 1965 WP_001026871.1 glycoside hydrolase family 127 protein -
JNN40_RS16035 3360748..3362148 - 1401 WP_000204815.1 MFS transporter -
JNN40_RS16040 3362374..3363189 + 816 WP_094931698.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6819.94 Da        Isoelectric Point: 12.0345

>T187911 WP_000833474.1 NZ_CP068802:3358089-3358274 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSRHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T187911 NZ_CP090126:694111-694452 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT187911 WP_000499746.1 NZ_CP068802:3358291-3358701 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT187911 NZ_CP090126:693773-694090 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167AMN3

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References