Detailed information of TA system TA187910

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3226927..3227759
• Replicon: chromosome
• Accession: NZ_CP068802
• Organism: Escherichia coli strain RIVM_C018249

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E3PN67
• Locus tag: JNN40_RS15415
• Protein ID: WP_000854775.1
• Coordinates: 3226927..3227301 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A426P7N5
• Locus tag: JNN40_RS15420
• Protein ID: WP_001285645.1
• Coordinates: 3227391..3227759 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN40_RS15385	3222286..3223209	-	924	WP_000535948.1	carboxylate/amino acid/amine transporter	-
JNN40_RS15390	3223320..3224504	-	1185	WP_001172857.1	sugar efflux transporter	-
JNN40_RS15395	3224935..3225063	-	129	Protein_2996	virulence RhuM family protein	-
JNN40_RS15400	3225303..3226145	-	843	WP_001274548.1	DUF4942 domain-containing protein	-
JNN40_RS15405	3226230..3226427	-	198	WP_000772035.1	DUF957 domain-containing protein	-
JNN40_RS15410	3226439..3226930	-	492	WP_000976825.1	hypothetical protein	-
JNN40_RS15415	3226927..3227301	-	375	WP_000854775.1	TA system toxin CbtA family protein	Toxin
JNN40_RS15420	3227391..3227759	-	369	WP_001285645.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN40_RS15425	3227922..3228143	-	222	WP_000692303.1	DUF987 domain-containing protein	-
JNN40_RS15430	3228212..3228688	-	477	WP_001318130.1	RadC family protein	-
JNN40_RS15435	3228704..3229189	-	486	WP_000213721.1	antirestriction protein	-
JNN40_RS15440	3229281..3230099	-	819	WP_001175173.1	DUF945 domain-containing protein	-
JNN40_RS15445	3230189..3230422	-	234	WP_001278284.1	DUF905 family protein	-
JNN40_RS15450	3230428..3231105	-	678	WP_001097567.1	hypothetical protein	-
JNN40_RS15455	3231256..3231936	-	681	WP_001282911.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13965.02 Da
• Isoelectric Point: 8.2830

>T187910 WP_000854775.1 NZ_CP068802:c3227301-3226927 [Escherichia coli]
MKTLPDTLVREASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T187910 NZ_CP090126:324422-324790 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCTAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13678.37 Da
• Isoelectric Point: 5.4492

>AT187910 WP_001285645.1 NZ_CP068802:c3227759-3227391 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTNG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT187910 NZ_CP090126:324204-324425 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: E3PN67

Antitoxin

• AlphaFold DB: A0A426P7N5