Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2693173..2693670 Replicon chromosome
Accession NZ_CP068802
Organism Escherichia coli strain RIVM_C018249

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN40_RS12965 Protein ID WP_001173343.1
Coordinates 2693173..2693346 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag JNN40_RS12970 Protein ID WP_001560287.1
Coordinates 2693374..2693670 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN40_RS12955 2689843..2691300 + 1458 WP_000856819.1 dipeptide/tripeptide permease DtpC -
JNN40_RS12960 2691537..2693054 + 1518 WP_000003806.1 lysine--tRNA ligase -
JNN40_RS12965 2693173..2693346 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN40_RS12970 2693374..2693670 - 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN40_RS12975 2693899..2694171 - 273 WP_000405647.1 N-acetyltransferase -
JNN40_RS12980 2694183..2694413 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
JNN40_RS12985 2694594..2696225 + 1632 WP_001216477.1 sensor histidine kinase -
JNN40_RS12990 2696222..2696941 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T187909 WP_001173343.1 NZ_CP068802:c2693346-2693173 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T187909 NZ_CP090125:2458447-2458626 [Bacillus subtilis]
ATGTCGACCTATGAATCTTTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT187909 WP_001560287.1 NZ_CP068802:c2693670-2693374 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT187909 NZ_CP090125:c2458773-2458550 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACAACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCATAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAGAAGAAACTACCCTTTAATA
GGAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References