Detailed information of TA system TA187904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2346984..2347471
• Replicon: chromosome
• Accession: NZ_CP068802
• Organism: Escherichia coli strain RIVM_C018249

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: JNN40_RS11325
• Protein ID: WP_001160961.1
• Coordinates: 2346984..2347235 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JNN40_RS11330
• Protein ID: WP_000125566.1
• Coordinates: 2347238..2347471 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN40_RS11300	2342596..2343882	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JNN40_RS11305	2343882..2344871	+	990	WP_000241249.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JNN40_RS11310	2344868..2345689	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JNN40_RS11315	2345692..2346069	+	378	WP_202859949.1	Co2+/Mg2+ efflux protein ApaG	-
JNN40_RS11320	2346076..2346924	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JNN40_RS11325	2346984..2347235	-	252	WP_001160961.1	CcdB family protein	Toxin
JNN40_RS11330	2347238..2347471	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JNN40_RS11335	2347557..2348036	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
JNN40_RS11340	2348228..2350090	-	1863	WP_000377114.1	glutathione-regulated potassium-efflux system protein KefC	-
JNN40_RS11345	2350083..2350613	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JNN40_RS11350	2350721..2352052	-	1332	WP_001183198.1	MFS transporter	-
JNN40_RS11355	2352111..2352398	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9512.19 Da
• Isoelectric Point: 10.4134

>T187904 WP_001160961.1 NZ_CP068802:c2347235-2346984 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T187904 NZ_CP090103:361924-362349 [Rhizobium leguminosarum]
ATGATTATCCTCGATACGAACGTTGTTTCCGAGGCGATGAAACCCACGCCCGACGAAGCCGTGAAATTCTGGCTCGATGA
GCAGGCAGCCGAAACCCTGTTCCTTTCTAGCGTCACGATCGCCGAACTGATGTTTGGAATCGGCGCGCTCCCGGCTGGCA
AGCGCAGGGAAAGGCTCTCCGACGCGTTGGACGGTCTGATGGAGCTCTTCGAAAGCCGTATTTTGGCTTTCGACATCACA
GCGGCACGGCACTATGCCGATCTCGCCGTCAAGGCCCGAGCCGCTGGCAGAGGATTCCCCACTCCGGATGGCTATATCGC
CGCCATCGCAGCATCAAAAGGGTTCGCCGTCGCCACGCGCGATACAAGTGCTTTCAACGCTGCGGGCGTTAAAGTGATCA
ATCCCTGGGCGGCTTCGACCGCATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT187904 WP_000125566.1 NZ_CP068802:c2347471-2347238 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT187904 NZ_CP090103:361673-361927 [Rhizobium leguminosarum]
ATGCCCGCAGTCACAATCAGAAACCTCTCCGAGGCGACGCACCGTGCTCTCAAGGTGCGTGCGGCTCATCACGGGCGCAG
CGCCGAAGCGGAAATGCGGGAAATCCTCGAGGCTGCCGTGCGTCCAGAAACGCGGCTTCGTCTTGGTAGCGCACTCGCCG
AACGGAGCCGCCGCCTCGGACTGACGAATGAGGACTTTGCCGTTCTGGACCAAGCTCGCGACACATTGCCTGCAAAGCCG
ATGAGCTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9