Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 1166853..1168573 Replicon chromosome
Accession NZ_CP068802
Organism Escherichia coli strain RIVM_C018249

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag JNN40_RS05795 Protein ID WP_000312833.1
Coordinates 1166853..1167509 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag JNN40_RS05800 Protein ID WP_000634203.1
Coordinates 1167506..1168573 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN40_RS05775 1162253..1162507 - 255 WP_154670580.1 hypothetical protein -
JNN40_RS05780 1162789..1163028 + 240 WP_001431807.1 hypothetical protein -
JNN40_RS05785 1163068..1165506 + 2439 WP_000985465.1 type I restriction-modification system subunit M -
JNN40_RS05790 1165506..1166840 + 1335 WP_000228013.1 restriction endonuclease subunit S -
JNN40_RS05795 1166853..1167509 + 657 WP_000312833.1 DUF4433 domain-containing protein Toxin
JNN40_RS05800 1167506..1168573 + 1068 WP_000634203.1 macro domain-containing protein Antitoxin
JNN40_RS05805 1168592..1171687 + 3096 WP_000108735.1 HsdR family type I site-specific deoxyribonuclease -
JNN40_RS05810 1171687..1172403 + 717 WP_001122107.1 M48 family metallopeptidase -
JNN40_RS05815 1172748..1173362 + 615 Protein_1142 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgB / csgD / csgE / csgF / csgG 1141214..1202491 61277


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T187901 WP_000312833.1 NZ_CP068802:1166853-1167509 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T187901 NZ_CP090101:39675-40583 [Rhizobium leguminosarum]
ATGGCGCGTGAACTCAAGAAAAACGTAGGGGCATCGGTGCGCGCCCGGCTGCTAGATCGGTCTCGCGCAGAGCGTACGGA
CTTTCAGATTCTACTGACGCGCTACGCGCTCGAAAGACTCCTGTACCGGCTGAGCCTATCGGAGCATCGCGACCGCTTTA
TCCTGAAGGGCGCGATGCTGTTCGTAACCTGGGTCGCCGACCCGTTTCGGCCGACGCGGGACCTCGATCTGCTGGGCTAC
GGCGAAAACAGCCCGAAGGCCATCGCCGAAACTTTCCATGCCATTTGCACGCAGCCGGTCGACGCTGATGGCGTAGTCTT
CGACGTGGATGCACTCGCAGCCGCGCCGATCCGTGAGGAAGTCGAATATGGCGGCGTTCGTGTCCGTACCACTGCCACCA
TCGGAGGCGCGCGCATTCCCATTCAGGTGGACATAGGTTTCGGTGACGCCGTCACGCCTGGACCGGTCGACATAGAATAT
CCGTCGCTGCTCGATGCGCCGGTGCCGCGCCTGCGGGCCTATCCGGTCGCGACCGTGGTCGCCGAGAAATTCGAGGCGCT
GGTATCGCTCGGCATGGCAAATAGCCGCCTGAAGGATTTCTATGATCTCTGGCTGATTGCCGAGACGTTCGAGTTGGAGC
GGTCCACCCTCGCTGAGGCGGTGCGCCAGACCTTTGCGAGACGAGGGACGAGTATTCCGACGGACAGGCCAACGGGTCTC
AGCGAAGCCTATGCTGAGGTCTGGGATCGGCAATGGCGGGCGTTCCTTGGTCGCGAACGGATGGCGGCCGCACCACCCGC
CTTGGGCACGGTGGTCGCTGATCTCGGACGCTTCCTATTGCCGCTGACGCAGCCTGTGGAAGGGGAATGGCGCTGGAAGC
CTCATGAGGGCTGGATTCAGCTCAAGTAA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT187901 WP_000634203.1 NZ_CP068802:1167506-1168573 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT187901 NZ_CP090101:39065-39685 [Rhizobium leguminosarum]
ATGACATTCACTACCGCCCAGAGACTTCGAGCGCTCGACCTCCTGAAAGCGCGGGGGATGCTCCGGCTGAAGGATTTCGT
TGCCGAAGGCATTGGACCGGAGACGCTCGCCCGTCTCGTTCGGGAGGAGGCAGTAGTCCGCCCGGCACGAGGCCTGTATC
AACTCCCGGACACTCAGGCTGAGGCGGCCCATGCACTCGCTGAAGCGGCTGTTCTGGTTCCAAAGGGCGTGGTCTGCTTG
ACCTCCGCCCTCCAATACCACGAACTGACGCTGCAGATGCCCTCGGCTGTGTGGATGGCGATCGACCGCACTGCCTGGCG
TCCCAAGATCGATTATCCGCCTATCAAGTTTGTACGCTTCAAGGGGTCCGCTCTCACCGAGGGCGTCGAACGGCATCGCA
TCGAGGGCGTCGAGGTGCCGATCACAAATCCGGCGCGTTCGATCGTCGATTGCTTCCGCTATCGGGCGAAAGTCGGCCTC
GATGTTGCGATGGAGGGCCTGCGCGAGGGGCTCCGTCGGCGCAAGACCACAAGCGACGACCTCTGGACATATGCGAAAGA
GGCGAGGATCTGGTCGATCATGCGACCCTATGTCGAAGCGACGGTGGCCGATGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References