Detailed information of TA system TA187900

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1149829..1150624
• Replicon: chromosome
• Accession: NZ_CP068802
• Organism: Escherichia coli strain RIVM_C018249

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: JNN40_RS05715
• Protein ID: WP_000854914.1
• Coordinates: 1149829..1150203 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JNN40_RS05720
• Protein ID: WP_074522479.1
• Coordinates: 1150250..1150624 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN40_RS05675	1144833..1145324	-	492	WP_032159295.1	DUF1097 domain-containing protein	-
JNN40_RS05680	1145426..1145980	-	555	WP_001001926.1	molecular chaperone YcdY	-
JNN40_RS05685	1146004..1146741	-	738	WP_000283664.1	zinc-binding phosphatase	-
JNN40_RS05690	1146796..1147734	-	939	WP_000351297.1	glyoxylate/hydroxypyruvate reductase GhrA	-
JNN40_RS05700	1148205..1149047	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
JNN40_RS05705	1149132..1149329	-	198	WP_000839282.1	DUF957 domain-containing protein	-
JNN40_RS05710	1149341..1149832	-	492	WP_000976857.1	hypothetical protein	-
JNN40_RS05715	1149829..1150203	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
JNN40_RS05720	1150250..1150624	-	375	WP_074522479.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JNN40_RS05725	1150787..1151008	-	222	WP_001220314.1	DUF987 domain-containing protein	-
JNN40_RS05730	1151071..1151547	-	477	WP_001186726.1	RadC family protein	-
JNN40_RS05735	1151563..1152048	-	486	WP_000214307.1	antirestriction protein	-
JNN40_RS05740	1152140..1152961	-	822	WP_001761104.1	DUF945 domain-containing protein	-
JNN40_RS05745	1153062..1153270	-	209	Protein_1128	DUF905 family protein	-
JNN40_RS05750	1153371..1153826	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	1141214..1202491	61277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T187900 WP_000854914.1 NZ_CP068802:c1150203-1149829 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T187900 NZ_CP090098:c2633044-2632751 [Rhizobium leguminosarum]
GTGAGGCTTGTCTGGGCGAGGTACGCGTTGTCCGACCGAGAGAATATTTTCACATATATTGAGATCGAGAATCCAAGGGC
GGCAGTTCATGTTGACCAGAAAATCGTTCTTGCTGTTCGCCGCCTCGTCGAATTCCCGGAAAGCGGAAGGCCGGGAAGAA
TCGCGGGCACACGGGAGCTGGTAATCCCAAGCACGCCTTACATCGCCGCGTATGTGGTGCATGAAGACCGAATCCGTATT
CTTCGCGTCCTCCACGGCGCCCAGATTTGGCCTGACGCCATATCAACCGACTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13783.63 Da
• Isoelectric Point: 6.6248

>AT187900 WP_074522479.1 NZ_CP068802:c1150624-1150250 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNLRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT187900 NZ_CP090098:c2633349-2633041 [Rhizobium leguminosarum]
ATGGCGTCAAACGCACTCGTTCAAACTCGCATAGATGCTGCGGTCAAGGATCGCGCCACGGCTGTTCTGGAAAACATGGG
CCTGACCGTCTCGGATGTCGTCCGTATTCTGTTGACCCGCACAGCGAATGAGGGAGCTCTTCCGCTGGAGCTTATCAGCA
ACAGTGACGCTTACGATGCGTGGTTTCGCGGGAAAGTCCTGGAAGCTCTCCATGACACGCGACCGGATGTTGACGATACC
GAGGTCGAGACCGGTTTTGAGAAGAGGCGCGAGGCGGCACTTCGGAAGAGCCAGGTGGATCGATCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5