Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4844851..4845348 Replicon chromosome
Accession NZ_CP068801
Organism Escherichia coli strain RIVM_C018220

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN52_RS23660 Protein ID WP_001173343.1
Coordinates 4844851..4845024 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN52_RS23665 Protein ID WP_000398619.1
Coordinates 4845052..4845348 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN52_RS23650 4841521..4842978 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN52_RS23655 4843215..4844732 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN52_RS23660 4844851..4845024 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN52_RS23665 4845052..4845348 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN52_RS23670 4845577..4845849 - 273 WP_000405647.1 N-acetyltransferase -
JNN52_RS23675 4845861..4846091 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN52_RS23680 4846271..4847902 + 1632 WP_001216477.1 sensor histidine kinase -
JNN52_RS23685 4847899..4848618 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4799974..4845348 45374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T187888 WP_001173343.1 NZ_CP068801:c4845024-4844851 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T187888 NZ_CP090095:1126096-1126473 [Neorhizobium galegae]
ATGGTGAGCGATAGCGCCGTTCTCGATACGAATATTATCGTCGATTATTTGAATTCCGTATCCGAGGGGCGTGAGGAATT
TGAACGATACGAAAATCCAAGCATCAGCATTGTGACATGGATGGAAGTGCTCGTCGGTGTGCCTCCACATCTCGCTGCGG
CAACACGAGAATTCCTTAATGCGCTGAATATCATCGCGCTTGACGATGCAGTTGCCGAAAGGGCCGTGTCCCTTCGCCAG
AAGCACAAGATGAAGCTTCCCGATGCGATCATCTGGGCGAGCGCGGATACTCACTCAATGCTGCTCGTCACAAGGAACAC
GAAGGACTTTCCTGCAAATCTGCCGGGAATTCGCGTACCTTACGTTTTGCGCCATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT187888 WP_000398619.1 NZ_CP068801:c4845348-4845052 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT187888 NZ_CP090095:1125887-1126102 [Neorhizobium galegae]
TTGCAGACCGTAGTCGAGTTGCCAGATGCTGAAGTCGAGGCATTGGACCGTTTGGCGCAGAAGGAGAACATATCTCGATC
TGTTCTGATCGAGCGTGCCGTGCACGATTTTCTGCAGAAAAGTGACAAGCAAAAGAGAGCCGAAGCTTTCGGCCTGTGGG
GTAATCGCATCGTCGATGGCTTGGACTTTCAGCAAAAAGTCCGCGATGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References