Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4481786..4482294 Replicon chromosome
Accession NZ_CP068801
Organism Escherichia coli strain RIVM_C018220

Toxin (Protein)


Gene name shpA Uniprot ID -
Locus tag JNN52_RS21745 Protein ID WP_001534843.1
Coordinates 4482085..4482294 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0V9L4L3
Locus tag JNN52_RS21740 Protein ID WP_000063161.1
Coordinates 4481786..4482088 (-) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN52_RS21725 4478314..4480464 + 2151 WP_001366791.1 pyruvate/proton symporter BtsT -
JNN52_RS21730 4480582..4480785 + 204 WP_001534846.1 YbdD/YjiX family protein -
JNN52_RS21735 4480796..4481752 + 957 WP_001534845.1 GTPase -
JNN52_RS21740 4481786..4482088 - 303 WP_000063161.1 BrnA antitoxin family protein Antitoxin
JNN52_RS21745 4482085..4482294 - 210 WP_001534843.1 BrnT family toxin Toxin
JNN52_RS21750 4482390..4483556 + 1167 WP_001534841.1 restriction endonuclease -
JNN52_RS21755 4483620..4485239 + 1620 WP_001550561.1 type I restriction-modification system subunit M -
JNN52_RS21760 4485229..4486539 + 1311 WP_001534837.1 restriction endonuclease subunit S -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(37-93)


Sequences


Toxin        


Download         Length: 70 a.a.        Molecular weight: 7952.17 Da        Isoelectric Point: 4.9493

>T187883 WP_001534843.1 NZ_CP068801:c4482294-4482085 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Download         Length: 210 bp

>T187883 NZ_CP090094:c187674-187381 [Rhizobium leguminosarum]
ATGAGTTTTAAGCTTTCTGCCGATGCGGAAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTATGTTCGGGGCAGG
TCAGGCAAAGCGATACCACGATGAACTTTTCGCGTTATTTGATCTGATAGCTGTCAATCCACGCATCGCGCGCGAACGGG
ATGAGATTGATCCGCCTGTTAGAATTCATCCCTTCAAAGCGCATCTAGTCGTTTATCGGATCGAAGATGATGAGACAATT
TTTGTAATACGAATTCGCCACGCCCATGAAGATTGGGCAACTGATTCAATTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11600.10 Da        Isoelectric Point: 9.4646

>AT187883 WP_000063161.1 NZ_CP068801:c4482088-4481786 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT187883 NZ_CP090094:c187913-187671 [Rhizobium leguminosarum]
ATGGCCACGATGAACGTATCTCTTCCAGGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAGGACCGGGCGATATTCTAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAGACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGGTGTCGGCATCCGATCAAAGGATGAACTCTTTGCTGAGGCGATAGCTCGTGCCAGCAAGTCA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0V9L4L3

References