Detailed information of TA system TA187877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3119575..3120359
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JNN52_RS15250
• Protein ID: WP_000613626.1
• Coordinates: 3119865..3120359 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JNN52_RS15245
• Protein ID: WP_001110447.1
• Coordinates: 3119575..3119868 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS15235	3114725..3115684	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JNN52_RS15240	3116257..3119442	+	3186	WP_001550951.1	ribonuclease E	-
JNN52_RS15245	3119575..3119868	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JNN52_RS15250	3119865..3120359	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JNN52_RS15255	3120454..3121407	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
JNN52_RS15260	3121419..3123062	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
JNN52_RS15265	3123128..3124069	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JNN52_RS15270	3124069..3125166	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T187877 WP_000613626.1 NZ_CP068801:3119865-3120359 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T187877 NZ_CP090081:2100500-2101237 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT187877 WP_001110447.1 NZ_CP068801:3119575-3119868 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT187877 NZ_CP090081:2101350-2101478 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6