Detailed information of TA system TA187876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2689881..2690519
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: JNN52_RS13045
• Protein ID: WP_000813795.1
• Coordinates: 2690343..2690519 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JNN52_RS13040
• Protein ID: WP_076797675.1
• Coordinates: 2689881..2690297 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS13020	2685033..2685974	-	942	WP_001251315.1	ABC transporter permease	-
JNN52_RS13025	2685975..2686988	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
JNN52_RS13030	2687006..2688151	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
JNN52_RS13035	2688396..2689802	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
JNN52_RS13040	2689881..2690297	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JNN52_RS13045	2690343..2690519	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JNN52_RS13050	2690741..2690971	+	231	WP_023910283.1	YncJ family protein	-
JNN52_RS13055	2691063..2693024	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JNN52_RS13060	2693097..2693633	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
JNN52_RS13065	2693686..2694897	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T187876 WP_000813795.1 NZ_CP068801:c2690519-2690343 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T187876 NZ_CP090079:255-737 [Klebsiella michiganensis]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT187876 WP_076797675.1 NZ_CP068801:c2690297-2689881 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT187876 NZ_CP090079:1-267 [Klebsiella michiganensis]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1