Detailed information of TA system TA187875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2609320..2610908
• Replicon: chromosome
• Accession: NZ_CP068801
• Organism: Escherichia coli strain RIVM_C018220

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: JNN52_RS12715
• Protein ID: WP_001551128.1
• Coordinates: 2609586..2610908 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JNN52_RS12710
• Protein ID: WP_001296726.1
• Coordinates: 2609320..2609586 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN52_RS12710	2609320..2609586	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JNN52_RS12715	2609586..2610908	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
JNN52_RS12720	2611108..2612460	-	1353	WP_001551127.1	SIR2 family protein	-
JNN52_RS12725	2612675..2613707	-	1033	Protein_2493	ISNCY family transposase	-
JNN52_RS12730	2614141..2615502	+	1362	Protein_2494	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2612675..2613493	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T187875 WP_001551128.1 NZ_CP068801:2609586-2610908 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T187875 NZ_CP090078:c5259639-5259298 [Klebsiella michiganensis]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGACGCTATATCTGTCGCCTGTGGCAGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT187875 WP_001296726.1 NZ_CP068801:2609320-2609586 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT187875 NZ_CP090078:c5259944-5259660 [Klebsiella michiganensis]
ATGACACCTTGCTTTGGTGCTCGTCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGATCGGGCCTGCGTTACCGG
TGAATTCAGTAACGCAGAATCTTTGAAGTTGGATGAAGTATTCACACGTTTTATCCGCCAAATGGAATCGATGCTAACCA
CTGGTGAAATGAATCCTCGCCATGCCCACAGTGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGC
AGTTGCGGCTACGTATACATCGCCGTTTACCCCACTCATCGTTAA

Structures

Toxin

• AlphaFold DB: A0A829LC11