Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4675714..4676211 Replicon chromosome
Accession NZ_CP068800
Organism Escherichia coli strain RIVM_C017997

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN45_RS22445 Protein ID WP_001173343.1
Coordinates 4675714..4675887 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JNN45_RS22450 Protein ID WP_000398619.1
Coordinates 4675915..4676211 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN45_RS22435 4672384..4673841 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
JNN45_RS22440 4674078..4675595 + 1518 WP_001550472.1 lysine--tRNA ligase -
JNN45_RS22445 4675714..4675887 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN45_RS22450 4675915..4676211 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN45_RS22455 4676440..4676712 - 273 WP_000405647.1 N-acetyltransferase -
JNN45_RS22460 4676724..4676954 - 231 WP_001550470.1 (4Fe-4S)-binding protein -
JNN45_RS22465 4677134..4678765 + 1632 WP_001216477.1 sensor histidine kinase -
JNN45_RS22470 4678762..4679481 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T187855 WP_001173343.1 NZ_CP068800:c4675887-4675714 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T187855 NZ_CP090065:c1587334-1586915 [Pectobacterium polonicum]
ATGACGGATATCTTGATCAACAAGAGTAGCACCATACAAAGATGCCTACGTCGTATTCGTGAGGAGTTCGATACTGAGGA
AGGGTTTCGTCAAAACTTCACCAAACAAGATTCCGTCATACTTAACATACAGCGTGCCTGCGAGGCCGCTATTGATATCG
CTAATTACCTTATCCGTATCAAGAAACTAGGCATACCACAGAGCAGCCGGGATAGCTTCGCGCTTCTCTCCGCCAATCAG
ATAATTACCATTGATCTGTCTGACAATTTACAAAAAATGGTGGGATTAAGAAATATTGCCGTACATGACTATCAGGCACT
TAACTTGGATATTGTCATTCACGTCATTTCCCATCGTTTAAGCGATTTTGAGCATTTTATCAAAGAAATAGTCCGATACA
GCGACAGCCAGCACCTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT187855 WP_000398619.1 NZ_CP068800:c4676211-4675915 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT187855 NZ_CP090065:c1587714-1587331 [Pectobacterium polonicum]
ATGTTCGACGAGATCGTTTCAACGCTGAAAAAACAGATTCCCGACATCAGGATGGTTTACCTTTTTGGCTCTCAGGCAAC
GGGCAACGCCAGAGCTGACAGCGATATTGATATCGCCATCATGGCGACGAGAACATTAGCTCCCGTTGAACGCTGGGAGT
TATCTAATCAGTTAGCAAAAGCAATGGGACGTGATGTCGATCTTGTCGATTTATTGCAGGCCTCAACGGTATTAAAAATG
GAGATCGTGCGTAATGGCAAGCTGCTTTATGATGCGGAAACCGCAGCGGGGGAATTTGAAATGATAACACTGTCGATGTA
CCAGCATCTGCAAAAAGAGCGTGCGGATATCATTCGTAGTTTTCATCAGGATCTAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References