Detailed information of TA system TA187849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4059032..4060721
• Replicon: chromosome
• Accession: NZ_CP068800
• Organism: Escherichia coli strain RIVM_C017997

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: JNN45_RS19455
• Protein ID: WP_000365809.1
• Coordinates: 4059032..4059952 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: JNN45_RS19460
• Protein ID: WP_001550661.1
• Coordinates: 4059939..4060721 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN45_RS19440	4055122..4056249	-	1128	WP_001550663.1	MFS transporter	-
JNN45_RS19445	4056391..4057449	-	1059	WP_122987197.1	alpha/beta hydrolase	-
JNN45_RS19450	4057714..4058598	+	885	WP_001119037.1	LysR family transcriptional regulator	-
JNN45_RS19455	4059032..4059952	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
JNN45_RS19460	4059939..4060721	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
JNN45_RS19465	4061037..4064246	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
JNN45_RS19470	4064407..4064547	+	141	WP_001308408.1	ash family protein	-
JNN45_RS19475	4064593..4065063	+	471	WP_000201185.1	hypothetical protein	-
JNN45_RS19480	4065060..4065162	+	103	Protein_3818	helix-turn-helix domain-containing protein	-
JNN45_RS19485	4065254..4065412	+	159	Protein_3819	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T187849 WP_000365809.1 NZ_CP068800:c4059952-4059032 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T187849 NZ_CP090065:c560807-560556 [Pectobacterium polonicum]
ATGCGTACAATTAGCTACAGTGAAGCCCGACAAAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCTAT
TCTTATCACTCGACAGAATGGTGAAGCCTGCGTGCTTATGTCTCTGGAAGAATATAATTCTCTAGAGGAAACTGCGTATC
TACTGCGTTCACCAGCCAATGCAAAAAGGCTGATGAATTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT187849 WP_001550661.1 NZ_CP068800:c4060721-4059939 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT187849 NZ_CP090065:c560559-560305 [Pectobacterium polonicum]
GTGAAGCTAATCTGGTCAGAAGAAGTATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGCAGAACACCTTTTGAAGGAAAAGGAAAACCAGAGCCACTTAAACATAACCTTGCTG
GTTTCTGGTCGCGCCGCATCACCGAAGAACACCGTCTGGTTTATGCCATCACCGATGACTCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Structures

Toxin

• AlphaFold DB: V0Z9M0