Detailed information of TA system TA187847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3449177..3449882
• Replicon: chromosome
• Accession: NZ_CP068800
• Organism: Escherichia coli strain RIVM_C017997

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: JNN45_RS16720
• Protein ID: WP_000539521.1
• Coordinates: 3449177..3449563 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JNN45_RS16725
• Protein ID: WP_001280945.1
• Coordinates: 3449553..3449882 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JNN45_RS16700	3445181..3445807	+	627	WP_001295292.1	glutathione S-transferase GstB	-
JNN45_RS16705	3445804..3446919	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
JNN45_RS16710	3447030..3447413	-	384	WP_000497137.1	biofilm formation regulator BssR	-
JNN45_RS16715	3447626..3448951	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
JNN45_RS16720	3449177..3449563	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JNN45_RS16725	3449553..3449882	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
JNN45_RS16730	3449952..3451280	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
JNN45_RS16735	3451288..3453636	-	2349	WP_001550886.1	EAL domain-containing protein	-
JNN45_RS16740	3453814..3454725	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T187847 WP_000539521.1 NZ_CP068800:3449177-3449563 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T187847 NZ_CP090065:405290-405577 [Pectobacterium polonicum]
ATGACCTATAAGTTAAAATTTGTGCCTTCTGCAATGAAGGAATGGAAAAAGTTAGGGCATCCTGTCCGAGAGCAGTTCAA
GAAAAAATTGGCTGAGCGTTTGGAAAATCCTCGAGTTCCTTCAGCCCAACTTCATGGCCGTAAAGACCAGTATAAAATTA
AACTGAAGTCTGCTGGTTACAGGCTGGTGTATTTGGTCCAGGATGAGGTCATTACTGTGATGGTTATGGGGGGTGGAAAG
CGGGAAGGTAGCCAGATTTATTCTGATACTAAAGGTCGTTCTGCTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT187847 WP_001280945.1 NZ_CP068800:3449553-3449882 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT187847 NZ_CP090065:405046-405300 [Pectobacterium polonicum]
ATGGCTTTTAATATCCTAACTAACACCGCTGCCAGCATCACTGAGTTGAAACGTGACCCAATGGGTACAGTACGGGCTGC
TGAAGGTGAAACTATCGCTATACTGAACCGGAATGAACCGGCCTTCTACTGTGTGCCCCCCGCCGTATTCGCTTATTTAA
TGGAACTGGCTGAAGACGCAGAACTGGGGAAAACCGTTGATGATCGGATGTCAGAATTAGCTGACGCGAAAGAGATTTCG
CTCGATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6