Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1045271..1045925 Replicon chromosome
Accession NZ_CP068800
Organism Escherichia coli strain RIVM_C017997

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JNN45_RS05055 Protein ID WP_000244781.1
Coordinates 1045518..1045925 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag JNN45_RS05050 Protein ID WP_001564007.1
Coordinates 1045271..1045537 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN45_RS05030 1041359..1042792 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
JNN45_RS05035 1042837..1043148 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
JNN45_RS05040 1043312..1043971 + 660 WP_000250274.1 hemolysin III family protein -
JNN45_RS05045 1044048..1045028 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
JNN45_RS05050 1045271..1045537 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
JNN45_RS05055 1045518..1045925 + 408 WP_000244781.1 protein YgfX Toxin
JNN45_RS05060 1045965..1046486 - 522 WP_001055874.1 flavodoxin FldB -
JNN45_RS05065 1046598..1047494 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JNN45_RS05070 1047519..1048229 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JNN45_RS05075 1048235..1049968 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T187833 WP_000244781.1 NZ_CP068800:1045518-1045925 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T187833 NZ_CP090061:c2774470-2774186 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT187833 WP_001564007.1 NZ_CP068800:1045271-1045537 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT187833 NZ_CP090061:c2774702-2774460 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References