Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4712420..4712917 Replicon chromosome
Accession NZ_CP068799
Organism Escherichia coli strain RIVM_C017887

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JNN46_RS22620 Protein ID WP_001173343.1
Coordinates 4712420..4712593 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag JNN46_RS22625 Protein ID WP_001312331.1
Coordinates 4712621..4712917 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN46_RS22610 4709090..4710547 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
JNN46_RS22615 4710784..4712301 + 1518 WP_000003805.1 lysine--tRNA ligase -
JNN46_RS22620 4712420..4712593 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JNN46_RS22625 4712621..4712917 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JNN46_RS22630 4713145..4713417 - 273 WP_000405647.1 N-acetyltransferase -
JNN46_RS22635 4713429..4713659 - 231 WP_000373291.1 (4Fe-4S)-binding protein -
JNN46_RS22640 4713840..4715471 + 1632 WP_001216466.1 sensor histidine kinase -
JNN46_RS22645 4715468..4716187 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T187826 WP_001173343.1 NZ_CP068799:c4712593-4712420 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T187826 NZ_CP090059:949269-949787 [Sphingomonas cannabina]
ATGTCCCGGCGCCCCTATGCCGCGCCGACGCCCATCACCGCTGATCACCGGCTGGACGGATTCGCCTGCGGCAAGTCGCC
GCTGGACGATTGGTTGAGAGCGCACGCGCTCGAGAACGAAGGCAAGGCGTCGCGCACCTATGTCGTCACGGCGCAAGCCG
GCGAGGACGCCGGTAACGTGGTCGCCTACTATACGCTCGCCTACGGCAGCGTCACACGGGAGGAGGTGCCGAAAAAGATC
CGGCAAGGCCTGCCCAATCCGGTTCCGGTGATGGTTCTGGGGCGTCTGGCTGTCCACCGGAACCACGGCGGCAAGGGCAT
CGGCGCGGGACTGCTCCGGGAGGCGATGCAACGCACCCTGGACGCGTCGCTGGTCGCCGGCTTGCGGGCACTGATCGTCC
ATGCGCTCGATGACGAAGCAGTGGGTTTCTATGCCCGATATGGTTTTCAGGTGTTTCCCGCCGGGACCAGAACGCTGTTC
CTGCCGGTCGAGACGTTGCGTCAGGCCGTTGCCGGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT187826 WP_001312331.1 NZ_CP068799:c4712917-4712621 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT187826 NZ_CP090059:948970-949254 [Sphingomonas cannabina]
ATGGCGGACGTACTGGAGCGGCCGAAGGCCGATACGACGATCACGATGCGCCTGCCCGTGCAGACTCGTGAGCTGATCGA
TCGCGCCGCGGCGACGATCGACAAGTCGCGTACCGAGTTCGTGCTCGAGAGCGCCCGGCAGCGCGCCATCGATGTGCTGC
TCGATCAGCGGGTGTTCCACCTCGATGCCGCAGCGAGCGAAGCCTTTGCGGACATCCTGGAGAAGCCGCCGGCACCGGTC
GAGGCGCTGCGCACGCTGGTGGCGAGCAAGTCGCCTTGGGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References