Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4242502..4243052 Replicon chromosome
Accession NZ_CP068799
Organism Escherichia coli strain RIVM_C017887

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag JNN46_RS20335 Protein ID WP_001160966.1
Coordinates 4242502..4242816 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag JNN46_RS20340 Protein ID WP_000125568.1
Coordinates 4242819..4243052 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JNN46_RS20310 4238177..4239463 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
JNN46_RS20315 4239463..4240452 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
JNN46_RS20320 4240449..4241270 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
JNN46_RS20325 4241273..4241650 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
JNN46_RS20330 4241657..4242505 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
JNN46_RS20335 4242502..4242816 - 315 WP_001160966.1 CcdB family protein Toxin
JNN46_RS20340 4242819..4243052 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
JNN46_RS20345 4243138..4243617 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
JNN46_RS20350 4243809..4245671 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
JNN46_RS20355 4245664..4246194 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
JNN46_RS20360 4246302..4247633 - 1332 WP_001183198.1 MFS transporter -
JNN46_RS20365 4247692..4247979 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T187818 WP_001160966.1 NZ_CP068799:c4242816-4242502 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T187818 NZ_CP090028:3082069-3082353 [Serratia ureilytica]
ATGACTTATAAGCTTGAATTCGAAGAACATGCGCTGAAGGAATTTAAAAAACTGTCCCCGATGATCCGGGAACAGTTCAA
AAAAAAGCTGGCATCGGTGCTGGTTAATCCCCACGTCCCCGCCAACAAACTCGCCGGTTTGCCCGATTGCTACAAAATCA
AACTCAGAGCCTCAGGCTTTCGTTTGGTATATCGCGTCATGGAAACGGAGATCGTGGTTCTGGTCCTGTCTGTGGGCAAA
CGCGAACGTTCAGCGGCTTACACGGCTGCCAAAAAGCGCCTTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT187818 WP_000125568.1 NZ_CP068799:c4243052-4242819 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT187818 NZ_CP090028:3081828-3082079 [Serratia ureilytica]
ATGACGGTTCAACCCATTCTTGCCGACTCGGCTGTCAGCATCAGCGATTTTAAGAAATCGCCCAACGCCGCCCTTAAAGA
GGCACAAGGGCAACCGGTCGCCGTCCTGACCAACGGTAAGATTTCGGGATATTACGTCTCTCCGGAAACCTGGGAGGCGA
TCGCGGAATACCTGGAAGATATCGAACTCGCTGAAATCGTTCGTTCACGGATGAACGGCAAACGCATTAAGGTCAATTTG
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References